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dc.contributor.author
Freitas, Cleverson D. T.  
dc.contributor.author
Leite, Hugo B.  
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Oliveira, João P. B.  
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Amaral, Jackson L.  
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Egito, Antônio S.  
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Vairo Cavalli, Sandra Elizabeth  
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Lobo, Marina D. P.  
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Monteiro Moreira, Ana C. O.  
dc.contributor.author
Ramos, Márcio V.  
dc.date.available
2018-08-08T15:31:07Z  
dc.date.issued
2016-09  
dc.identifier.citation
Freitas, Cleverson D. T.; Leite, Hugo B.; Oliveira, João P. B.; Amaral, Jackson L.; Egito, Antônio S.; et al.; Insights into milk-clotting activity of latex peptidases from Calotropis procera and Cryptostegia grandiflora; Elsevier Science; Food Research International; 87; 9-2016; 50-59  
dc.identifier.issn
0963-9969  
dc.identifier.uri
http://hdl.handle.net/11336/54557  
dc.description.abstract
Latex fractions from Calotropis procera, Cryptostegia grandiflora, Plumeria rubra, and Himatanthus drasticus were assayed in order to prospect for new plant peptidases with milk-clotting activities, for use as rennet alternatives. Only C. procera and C. grandiflora latex fractions exhibited proteolytic and milk-clotting activities, which were not affected by high concentrations of NaCl and CaCl2. However, pre-incubation of both samples at 75 °C for 10 min eliminated completely their activities. Both proteolytic fractions were able to hydrolyze k-casein and to produce peptides of 16 kDa, a similar SDS-PAGE profile to commercial chymosin. RP-HPLC and mass spectrometry analyses of the k-casein peptides showed that the peptidases from C. procera or C. grandiflora hydrolyzed k-casein similar to commercial chymosin. The cheeses made with both latex peptidases exhibited yields, dry masses, and soluble proteins similar to cheeses prepared with commercial chymosin. In conclusion, C. procera and C. grandiflora latex peptidases with the ability to coagulate milk can be used as alternatives to commercial animal chymosin in the cheese manufacturing process.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Casein  
dc.subject
Chymosin  
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Himatanthus Drasticus  
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Papain-Like Peptidase  
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Plumeria Rubra  
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Rennet  
dc.subject.classification
Alimentos y Bebidas  
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Otras Ingenierías y Tecnologías  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Insights into milk-clotting activity of latex peptidases from Calotropis procera and Cryptostegia grandiflora  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-08-08T15:00:04Z  
dc.journal.volume
87  
dc.journal.pagination
50-59  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Freitas, Cleverson D. T.. Federal University of Ceara; Brasil  
dc.description.fil
Fil: Leite, Hugo B.. Federal University of Ceara; Brasil  
dc.description.fil
Fil: Oliveira, João P. B.. Federal University of Ceara; Brasil  
dc.description.fil
Fil: Amaral, Jackson L.. Federal University of Ceara; Brasil  
dc.description.fil
Fil: Egito, Antônio S.. Brazilian Agricultural Research Corporation; Brasil  
dc.description.fil
Fil: Vairo Cavalli, Sandra Elizabeth. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
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Fil: Lobo, Marina D. P.. Universidade de Fortaleza; Brasil  
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Fil: Monteiro Moreira, Ana C. O.. Universidade de Fortaleza; Brasil  
dc.description.fil
Fil: Ramos, Márcio V.. Federal University of Ceara; Brasil  
dc.journal.title
Food Research International  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.foodres.2016.06.020  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0963996916302551