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dc.contributor.author
Scursoni, Alejandra M.
dc.contributor.author
Galluzzo, Laura
dc.contributor.author
Camarero, Sandra
dc.contributor.author
Pozzo, Norma
dc.contributor.author
Gabri, Mariano Rolando
dc.contributor.author
De Acosta, Cristina Mateo
dc.contributor.author
Vázquez, Ana María
dc.contributor.author
Alonso, Daniel Fernando
dc.contributor.author
De Davila, María Teresa G.
dc.date.available
2018-08-07T22:30:22Z
dc.date.issued
2010-01
dc.identifier.citation
Scursoni, Alejandra M.; Galluzzo, Laura; Camarero, Sandra; Pozzo, Norma; Gabri, Mariano Rolando; et al.; Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry; Alliance Communications Group Division Allen Press; Pediatric And Developmental Pathology; 13; 1; 1-2010; 18-23
dc.identifier.issn
1093-5266
dc.identifier.uri
http://hdl.handle.net/11336/54525
dc.description.abstract
Gangliosides are glycolipids present on the cell surface. The N-glycolylated ganglioside NeuGc-GM3 has been described in some neoplasms, such as breast carcinoma and melanoma, but is usually not detected in normal human cells. Our aim was to evaluate the presence of NeuGc-GM3 in Wilms tumor by immunohistochemistry. Postchemotherapy tumors were grouped into different histologic subtypes considering the main preserved component. Formalin-fixed, paraffin-embedded tumor samples were cut into 5-mm sections. The monoclonal antibody 14F7, a mouse IgG1 that specifically recognizes NeuGc-GM3, and a peroxidase-labeled polymer conjugated to secondary antibodies were used. Sections from breast carcinoma were employed as positive controls. Presence of NeuGc-GM3 was evident in 22 of 25 (88%) cases. The staining was stronger in the epithelial component, with a membrane pattern and cytoplasmic diffusion. The stromal component expressed cytoplasmic NeuGc-GM3 in cells with rhabdomyoblastic differentiation. Tubules of adjacent renal tissue were also positive, but no expression of NeuGc-GM3 was detected in nontumoral fetal kidney. Until now, the expression of N-glycolylated gangliosides in pediatric solid tumors has not been investigated. The present study evidenced the expression of NeuGc-GM3 in a high proportion of Wilms tumors, suggesting its potential utility as a specific target of immunotherapy.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Alliance Communications Group Division Allen Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Gangliosides
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Gm3
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Immunohistochemistry
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Immunotherapy
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N-Glycolyl Neuraminic Acid
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Nephroblastoma
dc.subject.classification
Patología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-30T17:59:42Z
dc.journal.volume
13
dc.journal.number
1
dc.journal.pagination
18-23
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Lawrence
dc.description.fil
Fil: Scursoni, Alejandra M.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
dc.description.fil
Fil: Galluzzo, Laura. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
dc.description.fil
Fil: Camarero, Sandra. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
dc.description.fil
Fil: Pozzo, Norma. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
dc.description.fil
Fil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: De Acosta, Cristina Mateo. Centro de Inmunología Molecular; Cuba
dc.description.fil
Fil: Vázquez, Ana María. Centro de Inmunología Molecular; Cuba
dc.description.fil
Fil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: De Davila, María Teresa G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
dc.journal.title
Pediatric And Developmental Pathology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.2350/08-10-0544.1
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.2350/08-10-0544.1
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