Artículo
Epigenetic activation of Sox2 gene in the developing vertebrate neural plate
Bouzas, Santiago Oscar
; Marini, Melisa Soledad
; Torres Zelada, Eliana; Buzzi, Ailín Leticia
; Morales Vicente, David A.; Strobl Mazulla, Pablo Hernan
Fecha de publicación:
06/2016
Editorial:
American Society for Cell Biology
Revista:
Molecular Biology Of The Cell
ISSN:
1059-1524
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
One of the earliest manifestations of neural induction is onset of expression of the neural marker Sox2, mediated by the activation of the enhancers N1 and N2. By using loss and gain of function, we find that Sox2 expression requires the activity of JmjD2A and the Msk1 kinase, which can respectively demethylate the repressive H3K9me3 mark and phosphorylate the activating H3S10 (H3S10ph) mark. Bimolecular fluorescence complementation reveals that the adaptor protein 14-3-3, known to bind to H3S10ph, interacts with JMJD2A and may be involved in its recruitment to regulatory regions of the Sox2 gene. Chromatin immunoprecipitation reveals dynamic binding of JMJD2A to the Sox2 promoter and N-1 enhancer at the time of neural plate induction. Finally, we show a clear temporal antagonism on the occupancy of H3K9me3 and H3S10ph modifications at the promoter of the Sox2 locus before and after the neural plate induction. Taken together, our results propose a series of epigenetic events necessary for the early activation of the Sox2 gene in neural progenitor cells.
Palabras clave:
Epigenetics
,
Neural Plate
,
Sox2
,
Jmjd2a
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(IFIBYNE)
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Citación
Bouzas, Santiago Oscar; Marini, Melisa Soledad; Torres Zelada, Eliana; Buzzi, Ailín Leticia; Morales Vicente, David A.; et al.; Epigenetic activation of Sox2 gene in the developing vertebrate neural plate; American Society for Cell Biology; Molecular Biology Of The Cell; 27; 12; 6-2016; 1921-1927
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