Artículo
Automated quantification of protein periodic nanostructures in fluorescence nanoscopy images: Abundance and regularity of neuronal spectrin membrane-associated skeleton
Barabas, Federico Martín
; Masullo, Luciano Andrés
; Bordenave, Martín Diego
; Giusti, Sebastian Alejandro
; Unsain, Nicolas
; Refojo, Damian
; Caceres, Alfredo Oscar
; Stefani, Fernando Daniel
Fecha de publicación:
12/2017
Editorial:
Nature Publishing Group
Revista:
Scientific Reports
ISSN:
0068-1261
e-ISSN:
2045-2322
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Fluorescence nanoscopy imaging permits the observation of periodic supramolecular protein structures in their natural environment, as well as the unveiling of previously unknown protein periodic structures. Deciphering the biological functions of such protein nanostructures requires systematic and quantitative analysis of large number of images under different experimental conditions and specific stimuli. Here we present a method and an open source software for the automated quantification of protein periodic structures in super-resolved images. Its performance is demonstrated by analyzing the abundance and regularity of the spectrin membrane-associated periodic skeleton (MPS) in hippocampal neurons of 2 to 40 days in vitro, imaged by STED and STORM nanoscopy. The automated analysis reveals that both the abundance and the regularity of the MPS increase over time and reach maximum plateau values after 14 DIV. A detailed analysis of the distributions of correlation coefficients provides indication of dynamical assembly and disassembly of the MPS.
Palabras clave:
Protein Self-Assembly
,
Super-Resolution
,
Neuron
,
Cytoskeleton
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Articulos(CIBION)
Articulos de CENTRO DE INVESTIGACIONES EN BIONANOCIENCIAS "ELIZABETH JARES ERIJMAN"
Articulos de CENTRO DE INVESTIGACIONES EN BIONANOCIENCIAS "ELIZABETH JARES ERIJMAN"
Articulos(IBIOBA - MPSP)
Articulos de INST. D/INV.EN BIOMED.DE BS AS-CONICET-INST. PARTNER SOCIEDAD MAX PLANCK
Articulos de INST. D/INV.EN BIOMED.DE BS AS-CONICET-INST. PARTNER SOCIEDAD MAX PLANCK
Articulos(INIMEC - CONICET)
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Citación
Barabas, Federico Martín; Masullo, Luciano Andrés; Bordenave, Martín Diego; Giusti, Sebastian Alejandro; Unsain, Nicolas; et al.; Automated quantification of protein periodic nanostructures in fluorescence nanoscopy images: Abundance and regularity of neuronal spectrin membrane-associated skeleton; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-10
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