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dc.contributor.author
Perrotta, Ramiro Martin  
dc.contributor.author
Prietto, Jimena  
dc.contributor.author
Alonso, Silvia del Valle  
dc.contributor.author
Chiaramoni, Nadia Silvia  
dc.date.available
2018-08-06T18:40:20Z  
dc.date.issued
2016-02  
dc.identifier.citation
Perrotta, Ramiro Martin; Prietto, Jimena; Alonso, Silvia del Valle; Chiaramoni, Nadia Silvia; DSPC Liposomes Improve Transport of L-cysteine and Reduce Metabolic Activity; ScienceDomain International; British Biotechnology Journal; 12; 4; 2-2016; 1-11  
dc.identifier.issn
2231-292  
dc.identifier.uri
http://hdl.handle.net/11336/54298  
dc.description.abstract
Aims: In this work, we developed and characterized liposomal formulations that encapsulate L-cysteine to study their further application in drug delivery and amino acid supplementation. The lipids used were 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC).Methodology: Encapsulation efficiency and amino acid release were determined. For biophysical characterization of the three formulations, the size, surface charge and surface packing were also studied. Cell viability was analyzed with MTT reagent after treatments with formulations ir order to study efficiency of these systems in induce changes in metabolism.Results: Results showed that L-cysteine interacts at the polar head level and that this interaction stabilizes the surface charge and prevents aggregation. We also determined the influence on cell metabolism in all formulations. The presence of L-cysteine in the DSPC formulation induced deeper changes in metabolism, evidencing that this formulation provides better transport of this amino acid.Conclusion: Liposomes developed herein are well suited for the application in the delivery of L-cysteine. Particularly, they can encapsulate nearly all the L-cysteine and can retain it for 6 hours. Also, L-cysteine stabilized liposomes, preventing their aggregation. L-cysteine encapsulated in the DSPC formulation induced deeper changes in cell metabolism, causing a decrease in metabolic activity; this was probably due to a higher entry, thus a better liposome-mediated transport. Considering that the smaller the particle, the better the circulation, we believe that the stabilization of the vesicle by L-cysteine may allow these transporters to have higher circulation times. Based on the above, we conclude that the DSPC formulation is the best suited for further application in L-cysteine delivery.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
ScienceDomain International  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Liposomes  
dc.subject
L-Cysteine  
dc.subject
Ftir  
dc.subject
Cell Metabolism  
dc.subject.classification
Nano-materiales  
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Nanotecnología  
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS  
dc.title
DSPC Liposomes Improve Transport of L-cysteine and Reduce Metabolic Activity  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-08-03T14:17:39Z  
dc.journal.volume
12  
dc.journal.number
4  
dc.journal.pagination
1-11  
dc.journal.pais
Reino Unido  
dc.description.fil
Fil: Perrotta, Ramiro Martin. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Prietto, Jimena. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina  
dc.description.fil
Fil: Alonso, Silvia del Valle. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Chiaramoni, Nadia Silvia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.journal.title
British Biotechnology Journal  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.9734/BBJ/2016/24723  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://sciencedomain.org/abstract/13874