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dc.contributor.author
Menestrina, Fiorella  
dc.contributor.author
Osorio Grisales, Jaiver  
dc.contributor.author
Castells, Cecilia Beatriz Marta  
dc.date.available
2018-08-06T18:29:43Z  
dc.date.issued
2016-09  
dc.identifier.citation
Menestrina, Fiorella; Osorio Grisales, Jaiver; Castells, Cecilia Beatriz Marta; Chiral analysis of derivatized amino acids from kefir by gas chromatography; Elsevier Science; Microchemical Journal; 128; 9-2016; 267-273  
dc.identifier.issn
0026-265X  
dc.identifier.uri
http://hdl.handle.net/11336/54293  
dc.description.abstract
The amino acids present in the kefir samples were derivatized using different alkyl chloroformates combined with alcohols and chromatographed on a (3-O-butanoyl-2,6-di-O-pentyl)-γ-cyclodextrin (Lipodex E) chiral gas-liquid chromatographic column. The combination of derivatization reagents that led to better chromato- graphic performance for standard amino acid solutions was critically compared. All the chloroformate/alchohol mixtures lead to fast and quantitative reactions. However, the ethyl chloroformate/ethanolmixturewas selected since the number of enantioseparated amino acid derivativeswas higher. The derivatizationwas applied to samples of kefir grains grown in raw and in skimmed UHT milk. The samples were previously pretreated by elution froma low-pressure ion-exchange columns. Standard addition calibration curves have been used for the quantitative determinations of amino acid derivatives in both samples, and the figs. of merit of the proposed method have been assessed. The L-and D-configuration of D-and L-alanine, D-and L-valine,D-proline, L-threonine, aspartic and glutamic acids, methionine, and cysteine were detected and quantitatively determined.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Amino Acids Analysis  
dc.subject
Chiral  
dc.subject
Gas Chromatography  
dc.subject
Kefir  
dc.subject.classification
Otras Ciencias Químicas  
dc.subject.classification
Ciencias Químicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Chiral analysis of derivatized amino acids from kefir by gas chromatography  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-08-06T17:44:14Z  
dc.journal.volume
128  
dc.journal.pagination
267-273  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Menestrina, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina  
dc.description.fil
Fil: Osorio Grisales, Jaiver. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina  
dc.description.fil
Fil: Castells, Cecilia Beatriz Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina  
dc.journal.title
Microchemical Journal  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.microc.2016.05.007  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X16300595