Artículo
Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes
Roeschlin, Roxana Andrea
; Favaro, María Alejandra
; Chiesa, Maria Amalia
; Alemano, Sergio Gabriel; Vojnov, Adrián Alberto
; Castagnaro, Atilio Pedro
; Filippone, María Paula
; Gmitter, Frederick G.; Gadea, José; Marano, María Rosa
Fecha de publicación:
12/2017
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Molecular Plant Pathology
ISSN:
1464-6722
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Xanthomonas citri ssp. citri (X. citri) is the causal agent of Asiatic citrus canker, a disease that seriously affects most commercially important Citrus species worldwide. We have identified previously a natural variant, X. citri AT, that triggers a host-specific defence response in Citrus limon. However, the mechanisms involved in this canker disease resistance are unknown. In this work, the defence response induced by X. citri AT was assessed by transcriptomic, physiological and ultrastructural analyses, and the effects on bacterial biofilm formation were monitored in parallel. We show that X. citri AT triggers a hypersensitive response associated with the interference of biofilm development and arrest of bacterial growth in C. limon. This plant response involves an extensive transcriptional reprogramming, setting in motion cell wall reinforcement, the oxidative burst and the accumulation of salicylic acid (SA) and phenolic compounds. Ultrastructural analyses revealed subcellular changes involving the activation of autophagy-associated vacuolar processes. Our findings show the activation of SA-dependent defence in response to X. citri AT and suggest a coordinated regulation between the SA and flavonoid pathways, which is associated with autophagy mechanisms that control pathogen invasion in C. limon. Furthermore, this defence response protects C. limon plants from disease on subsequent challenges by pathogenic X. citri. This knowledge will allow the rational exploitation of the plant immune system as a biotechnological approach for the management of the disease.
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Identificadores
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(IICAR)
Articulos de INST. DE INVESTIGACIONES EN CIENCIAS AGRARIAS DE ROSARIO
Articulos de INST. DE INVESTIGACIONES EN CIENCIAS AGRARIAS DE ROSARIO
Citación
Roeschlin, Roxana Andrea; Favaro, María Alejandra; Chiesa, Maria Amalia; Alemano, Sergio Gabriel; Vojnov, Adrián Alberto; et al.; Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes; Wiley Blackwell Publishing, Inc; Molecular Plant Pathology; 18; 9; 12-2017; 1267-1281
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