Show simple item record

dc.contributor.author Camberos, Maria del Carmen
dc.contributor.author Perez, Adriana A.
dc.contributor.author Passicot, Gisel Anabel
dc.contributor.author Martucci, Lucia Camila
dc.contributor.author Wanderley, Maria
dc.contributor.author Udrisar, Daniel P.
dc.contributor.author Cresto, Juan Carlos
dc.date.available 2018-08-03T20:01:39Z
dc.date.issued 2016-10
dc.identifier.citation Camberos, Maria del Carmen; Perez, Adriana A.; Passicot, Gisel Anabel; Martucci, Lucia Camila; Wanderley, Maria; et al.; II - Insulin processing in mitochondria; Springer/Plenum Publishers; Journal of Bioenergetics and Biomembranes; 48; 5; 10-2016; 469-482
dc.identifier.issn 0145-479X
dc.identifier.uri http://hdl.handle.net/11336/54152
dc.description.abstract Our objective was to know how insulin is processing in mitochondria; if IDE is the only participant in mitochondrial insulin degradation and the role of insulin degradation on IDE accumulation in mitoplasts. Mitochondria and its fractions were isolated as described by Greenwalt. IDE was purified and detected in immunoblot with specific antibodies. High insulin degradation was obtained through addition to rat’s diet of 25�g/rat of apple and 10�g/rat of hard-boiled eggs, 3�days a week. Mitochondrial insulin degradation was assayed with 5�% TCA, insulin antibody or Sephadex G50 chromatography. Degradation was also assayed 60�min at 37��C in mitochondrial fractions (IMS and Mx) with diet or not and without IDE. Degradation in fractions precipitated with ammonium sulfates (60–80�%) were studied after mitochondrial insulin incubation (1�ng. insulin during 15�min, at 30��C) or with addition of 2.5�mM ATP. Supplementary diet increased insulin degradation. High insulin did not increase mitoplasts accumulation and did not decrease mitochondrial degradation. High insulin and inhibition of degradation evidence insulin competition for a putative transport system. Mitochondrial incubation with insulin increased IDE in matrix as observed in immunoblot. ATP decreased degradation in Mx and increased it in IMS. Chromatography of IMS demonstrated an ATP-dependent protease that degraded insulin, similar to described by Sitte et al. Mitochondria participate in insulin degradation and the diet increased it. High insulin did not accomplish mitochondrial decrease of degradation or its accumulation in mitoplasts. Mitochondrial incubation with insulin increased IDE in matrix. ATP suggested being a regulator of mitochondrial insulin degradation.
dc.format application/pdf
dc.language.iso eng
dc.publisher Springer/Plenum Publishers
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ATP-DEPENDENT PROTEASE
dc.subject CHROMATOGRAPHY STUDIES
dc.subject IDE INSULIN DEGRADATION
dc.subject MITOCHONDRIAL FRACTIONS
dc.subject SUPPLEMENTARY DIET
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title II - Insulin processing in mitochondria
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-07-05T13:37:42Z
dc.journal.volume 48
dc.journal.number 5
dc.journal.pagination 469-482
dc.journal.pais Estados Unidos
dc.journal.ciudad Nueva York
dc.description.fil Fil: Camberos, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
dc.description.fil Fil: Perez, Adriana A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
dc.description.fil Fil: Passicot, Gisel Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
dc.description.fil Fil: Martucci, Lucia Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
dc.description.fil Fil: Wanderley, Maria. Universidade Federal de Pernambuco; Brasil
dc.description.fil Fil: Udrisar, Daniel P.. Universidade Federal de Pernambuco; Brasil
dc.description.fil Fil: Cresto, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
dc.journal.title Journal of Bioenergetics and Biomembranes
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s10863-016-9682-8
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10863-016-9682-8
dc.conicet.fuente unificacion


Archivos asociados

Icon
Blocked Acceso no disponible

This item appears in the following Collection(s)

  • Articulos(CEDIE) [99]
    Articulos de CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"

Show simple item record

info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)