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dc.contributor.author
Camberos, Maria del Carmen
dc.contributor.author
Perez, Adriana A.
dc.contributor.author
Passicot, Gisel Anabel
dc.contributor.author
Martucci, Lucia Camila
dc.contributor.author
Wanderley, Maria
dc.contributor.author
Udrisar, Daniel P.
dc.contributor.author
Cresto, Juan Carlos
dc.date.available
2018-08-03T20:01:39Z
dc.date.issued
2016-10
dc.identifier.citation
Camberos, Maria del Carmen; Perez, Adriana A.; Passicot, Gisel Anabel; Martucci, Lucia Camila; Wanderley, Maria; et al.; II - Insulin processing in mitochondria; Springer/Plenum Publishers; Journal of Bioenergetics and Biomembranes; 48; 5; 10-2016; 469-482
dc.identifier.issn
0145-479X
dc.identifier.uri
http://hdl.handle.net/11336/54152
dc.description.abstract
Our objective was to know how insulin is processing in mitochondria; if IDE is the only participant in mitochondrial insulin degradation and the role of insulin degradation on IDE accumulation in mitoplasts. Mitochondria and its fractions were isolated as described by Greenwalt. IDE was purified and detected in immunoblot with specific antibodies. High insulin degradation was obtained through addition to rat’s diet of 25�g/rat of apple and 10�g/rat of hard-boiled eggs, 3�days a week. Mitochondrial insulin degradation was assayed with 5�% TCA, insulin antibody or Sephadex G50 chromatography. Degradation was also assayed 60�min at 37��C in mitochondrial fractions (IMS and Mx) with diet or not and without IDE. Degradation in fractions precipitated with ammonium sulfates (60–80�%) were studied after mitochondrial insulin incubation (1�ng. insulin during 15�min, at 30��C) or with addition of 2.5�mM ATP. Supplementary diet increased insulin degradation. High insulin did not increase mitoplasts accumulation and did not decrease mitochondrial degradation. High insulin and inhibition of degradation evidence insulin competition for a putative transport system. Mitochondrial incubation with insulin increased IDE in matrix as observed in immunoblot. ATP decreased degradation in Mx and increased it in IMS. Chromatography of IMS demonstrated an ATP-dependent protease that degraded insulin, similar to described by Sitte et al. Mitochondria participate in insulin degradation and the diet increased it. High insulin did not accomplish mitochondrial decrease of degradation or its accumulation in mitoplasts. Mitochondrial incubation with insulin increased IDE in matrix. ATP suggested being a regulator of mitochondrial insulin degradation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer/Plenum Publishers
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Atp-Dependent Protease
dc.subject
Chromatography Studies
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Ide Insulin Degradation
dc.subject
Mitochondrial Fractions
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Supplementary Diet
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
II - Insulin processing in mitochondria
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-05T13:37:42Z
dc.journal.volume
48
dc.journal.number
5
dc.journal.pagination
469-482
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Camberos, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
dc.description.fil
Fil: Perez, Adriana A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
dc.description.fil
Fil: Passicot, Gisel Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
dc.description.fil
Fil: Martucci, Lucia Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
dc.description.fil
Fil: Wanderley, Maria. Universidade Federal de Pernambuco; Brasil
dc.description.fil
Fil: Udrisar, Daniel P.. Universidade Federal de Pernambuco; Brasil
dc.description.fil
Fil: Cresto, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
dc.journal.title
Journal of Bioenergetics and Biomembranes
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s10863-016-9682-8
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10863-016-9682-8


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