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dc.contributor.author
Camberos, Maria del Carmen  
dc.contributor.author
Perez, Adriana A.  
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Passicot, Gisel Anabel  
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Martucci, Lucia Camila  
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Wanderley, Maria  
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Udrisar, Daniel P.  
dc.contributor.author
Cresto, Juan Carlos  
dc.date.available
2018-08-03T20:01:39Z  
dc.date.issued
2016-10  
dc.identifier.citation
Camberos, Maria del Carmen; Perez, Adriana A.; Passicot, Gisel Anabel; Martucci, Lucia Camila; Wanderley, Maria; et al.; II - Insulin processing in mitochondria; Springer/Plenum Publishers; Journal of Bioenergetics and Biomembranes; 48; 5; 10-2016; 469-482  
dc.identifier.issn
0145-479X  
dc.identifier.uri
http://hdl.handle.net/11336/54152  
dc.description.abstract
Our objective was to know how insulin is processing in mitochondria; if IDE is the only participant in mitochondrial insulin degradation and the role of insulin degradation on IDE accumulation in mitoplasts. Mitochondria and its fractions were isolated as described by Greenwalt. IDE was purified and detected in immunoblot with specific antibodies. High insulin degradation was obtained through addition to rat’s diet of 25�g/rat of apple and 10�g/rat of hard-boiled eggs, 3�days a week. Mitochondrial insulin degradation was assayed with 5�% TCA, insulin antibody or Sephadex G50 chromatography. Degradation was also assayed 60�min at 37��C in mitochondrial fractions (IMS and Mx) with diet or not and without IDE. Degradation in fractions precipitated with ammonium sulfates (60–80�%) were studied after mitochondrial insulin incubation (1�ng. insulin during 15�min, at 30��C) or with addition of 2.5�mM ATP. Supplementary diet increased insulin degradation. High insulin did not increase mitoplasts accumulation and did not decrease mitochondrial degradation. High insulin and inhibition of degradation evidence insulin competition for a putative transport system. Mitochondrial incubation with insulin increased IDE in matrix as observed in immunoblot. ATP decreased degradation in Mx and increased it in IMS. Chromatography of IMS demonstrated an ATP-dependent protease that degraded insulin, similar to described by Sitte et al. Mitochondria participate in insulin degradation and the diet increased it. High insulin did not accomplish mitochondrial decrease of degradation or its accumulation in mitoplasts. Mitochondrial incubation with insulin increased IDE in matrix. ATP suggested being a regulator of mitochondrial insulin degradation.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer/Plenum Publishers  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Atp-Dependent Protease  
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Chromatography Studies  
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Ide Insulin Degradation  
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Mitochondrial Fractions  
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Supplementary Diet  
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Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
II - Insulin processing in mitochondria  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
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info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-07-05T13:37:42Z  
dc.journal.volume
48  
dc.journal.number
5  
dc.journal.pagination
469-482  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Nueva York  
dc.description.fil
Fil: Camberos, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina  
dc.description.fil
Fil: Perez, Adriana A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina  
dc.description.fil
Fil: Passicot, Gisel Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina  
dc.description.fil
Fil: Martucci, Lucia Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina  
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Fil: Wanderley, Maria. Universidade Federal de Pernambuco; Brasil  
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Fil: Udrisar, Daniel P.. Universidade Federal de Pernambuco; Brasil  
dc.description.fil
Fil: Cresto, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina  
dc.journal.title
Journal of Bioenergetics and Biomembranes  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s10863-016-9682-8  
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info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10863-016-9682-8