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dc.contributor.author
Polat, Meripet
dc.contributor.author
Takeshima, Shin-nosuke
dc.contributor.author
Hosomichi, Kazuyoshi
dc.contributor.author
Kim, Jiyun
dc.contributor.author
Miyasaka, Taku
dc.contributor.author
Yamada, Kazunori
dc.contributor.author
Arainga, Mariluz
dc.contributor.author
Murakami, Tomoyuki
dc.contributor.author
Matsumoto, Yuki
dc.contributor.author
de la Barra Diaz, Verónica
dc.contributor.author
Panei, Carlos Javier
dc.contributor.author
Gonzalez, Ester Teresa
dc.contributor.author
Kanemaki, Misao
dc.contributor.author
Onuma, Misao
dc.contributor.author
Giovambattista, Guillermo
dc.contributor.author
Aida, Yoko
dc.date.available
2018-08-02T20:27:57Z
dc.date.issued
2016-01
dc.identifier.citation
Polat, Meripet; Takeshima, Shin-nosuke; Hosomichi, Kazuyoshi; Kim, Jiyun; Miyasaka, Taku; et al.; A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis; BioMed Central; Retrovirology; 13; 1; 1-2016; 1-23
dc.identifier.issn
1742-4690
dc.identifier.uri
http://hdl.handle.net/11336/53978
dc.description.abstract
Background: Bovine leukemia virus (BLV) is a member of retroviridae family, together with human T cell leukemia virus types 1 and 2 (HTLV-1 and -2) belonging to the genes deltaretrovirus, and infects cattle worldwide. Previous studies have classified the env sequences of BLV provirus from different geographic locations into eight genetic groups. To investigate the genetic variability of BLV in South America, we performed phylogenetic analyses of whole genome and partial env gp51 sequences of BLV strains isolated from Peru, Paraguay and Bolivia, for which no the molecular characteristics of BLV have previously been published, and discovered a novel BLV genotype, genotype-9, in Bolivia. Results: In Peru and Paraguay, 42.3 % (139/328) and over 50 % (76/139) of samples, respectively, were BLV positive. In Bolivia, the BLV infection rate was up to 30 % (156/507) at the individual level. In Argentina, 325/420 samples were BLV positive, with a BLV prevalence of 77.4 % at the individual level and up to 90.9 % at herd level. By contrast, relatively few BLV positive samples were detected in Chile, with a maximum of 29.1 % BLV infection at the individual level. We performed phylogenetic analyses using two different approaches, maximum likelihood (ML) tree and Bayesian inference, using 35 distinct partial env gp51 sequences from BLV strains isolated from Peru, Paraguay, and Bolivia, and 74 known BLV strains, representing eight different BLV genotypes from various geographical locations worldwide. The results indicated that Peruvian and Paraguayan BLV strains were grouped into genotypes-1, -2, and -6, while those from Bolivia were clustered into genotypes-1, -2, and -6, and a new genotype, genotype-9. Interestingly, these results were confirmed using ML phylogenetic analysis of whole genome sequences obtained by next generation sequencing of 25 BLV strains, assigned to four different genotypes (genotypes-1, -2, -6, and -9) from Peru, Paraguay, and Bolivia. Comparative analyses of complete genome sequences clearly showed some specific substitutions, in both structural and non-structural BLV genes, distinguishing the novel genotype-9 from known genotypes. Conclusions: Our results demonstrate widespread BLV infection in South American cattle and the existence of a new BLV genotype-9 in Bolivia. We conclude that at least seven BLV genotypes (genotypes-1, -2, -4, -5, -6, -7, and -9) are circulating in South America.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Blv Whole Genome Analysis
dc.subject
Bovine Leukemia Virus (Blv)
dc.subject
Gp51 Env Sequencing
dc.subject
Next Generation Sequencing
dc.subject
Phylogenetic Analysis
dc.subject
South America
dc.subject.classification
Virología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-31T17:20:25Z
dc.journal.volume
13
dc.journal.number
1
dc.journal.pagination
1-23
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Polat, Meripet. University of Tokyo; Japón. RIKEN; Japón
dc.description.fil
Fil: Takeshima, Shin-nosuke. University of Tokyo; Japón. RIKEN; Japón
dc.description.fil
Fil: Hosomichi, Kazuyoshi. Kanazawa University; Japón
dc.description.fil
Fil: Kim, Jiyun. RIKEN; Japón
dc.description.fil
Fil: Miyasaka, Taku. RIKEN; Japón
dc.description.fil
Fil: Yamada, Kazunori. RIKEN; Japón
dc.description.fil
Fil: Arainga, Mariluz. RIKEN; Japón
dc.description.fil
Fil: Murakami, Tomoyuki. RIKEN; Japón
dc.description.fil
Fil: Matsumoto, Yuki. RIKEN; Japón
dc.description.fil
Fil: de la Barra Diaz, Verónica. Laboratorio Veterinario Lavet; Chile
dc.description.fil
Fil: Panei, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
dc.description.fil
Fil: Gonzalez, Ester Teresa. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
dc.description.fil
Fil: Kanemaki, Misao. Institute for Animal Science; Japón
dc.description.fil
Fil: Onuma, Misao. RIKEN; Japón
dc.description.fil
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - La Plata. Instituto de Genética Veterinaria Ing. Fernando Noel Dulout; Argentina
dc.description.fil
Fil: Aida, Yoko. University of Tokyo; Japón. RIKEN; Japón
dc.journal.title
Retrovirology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://retrovirology.biomedcentral.com/articles/10.1186/s12977-016-0239-z
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s12977-016-0239-z


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