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dc.contributor.author Benavidez, Tomás Enrique
dc.contributor.author Torrente, Daniel
dc.contributor.author Marucho, Marcelo
dc.contributor.author Garcia, Carlos D
dc.date.available 2018-08-01T19:19:27Z
dc.date.issued 2015-03
dc.identifier.citation Benavidez, Tomás Enrique; Torrente, Daniel; Marucho, Marcelo; Garcia, Carlos D; Adsorption of soft and hard proteins onto OTCEs under the influence of an external electric field; American Chemical Society; Langmuir; 31; 8; 3-2015; 2455-2462
dc.identifier.issn 0743-7463
dc.identifier.uri http://hdl.handle.net/11336/53811
dc.description.abstract The adsorption behavior of hard and soft proteins under the effect of an external electric field was investigated by a combination of spectroscopic ellipsometry and molecular dynamics (MD) simulations. Optically transparent carbon electrodes (OTCE) were used as conductive, sorbent substrates. Lysozyme (LSZ) and ribonuclease A (RNase A) were selected as representative hard proteins, whereas myoglobin (Mb), α-lactalbumin (α-LAC), bovine serum albumin (BSA), glucose oxidase (GOx), and immunoglobulin G (IgG) were selected to represent soft proteins. In line with recent publications from our group, the experimental results revealed that while the adsorption of all investigated proteins can be enhanced by the potential applied to the electrode, the effect is more pronounced for hard proteins. In contrast with the incomplete monolayers formed at open-circuit potential, the application of +800 mV to the sorbent surface induced the formation of multiple layers of protein. These results suggest that this effect can be related to the intrinsic polarizability of the protein (induction of dipoles), the resulting surface accessible solvent area (SASA), and structural rearrangements induced upon the incorporation on the protein layer. The described experiments are critical to understand the relationship between the structure of proteins and their tendency to form (under electric stimulation) layers with thicknesses that greatly surpass those obtained at open-circuit conditions.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Chemical Society
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject PROTEIN ADSORPTION
dc.subject SOFT PROTEINS
dc.subject HARD PROTEINS
dc.subject OTCE
dc.subject.classification Otras Ciencias Químicas
dc.subject.classification Ciencias Químicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Adsorption of soft and hard proteins onto OTCEs under the influence of an external electric field
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-07-30T15:24:18Z
dc.identifier.eissn 1520-5827
dc.journal.volume 31
dc.journal.number 8
dc.journal.pagination 2455-2462
dc.journal.pais Estados Unidos
dc.journal.ciudad Washington DC
dc.description.fil Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. University of Texas; Estados Unidos
dc.description.fil Fil: Torrente, Daniel. University of Texas; Estados Unidos
dc.description.fil Fil: Marucho, Marcelo. University of Texas; Estados Unidos
dc.description.fil Fil: Garcia, Carlos D. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. University of Texas; Estados Unidos
dc.journal.title Langmuir
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/la504890v
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/la504890v
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433030/
dc.conicet.fuente individual


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  • Articulos(INFIQC) [800]
    Articulos de INST.DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA

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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)