Mostrar el registro sencillo del ítem
dc.contributor.author
Uribe, Rosa A.
dc.contributor.author
Buzzi, Ailín Leticia
dc.contributor.author
Bronner, Marianne E.
dc.contributor.author
Strobl Mazulla, Pablo Hernan
dc.date.available
2018-07-31T20:30:07Z
dc.date.issued
2015-11
dc.identifier.citation
Uribe, Rosa A.; Buzzi, Ailín Leticia; Bronner, Marianne E.; Strobl Mazulla, Pablo Hernan; Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression; Rockefeller University Press; Journal of Cell Biology; 211; 4; 11-2015; 815-827
dc.identifier.issn
0021-9525
dc.identifier.uri
http://hdl.handle.net/11336/53679
dc.description.abstract
In vertebrates, the inner ear arises from the otic placode, a thickened swathe of ectoderm that invaginates to form the otic vesicle. We report that histone demethylase KDM4B is dynamically expressed during early stages of chick inner ear formation. A loss of KDM4B results in defective invagination and striking morphological changes in the otic epithelium, characterized by abnormal localization of adhesion and cytoskeletal molecules and reduced expression of several inner ear markers, including Dlx3. In vivo chromatin immunoprecipitation reveals direct and dynamic occupancy of KDM4B and its target, H3K9me3, at regulatory regions of the Dlx3 locus. Accordingly, coelectroporations of DLX3 or KDM4B encoding constructs, but not a catalytically dead mutant of KDM4B, rescue the ear invagination phenotype caused by KDM4B knockdown. Moreover, a loss of DLX3 phenocopies a loss of KDM4B. Collectively, our findings suggest that KDM4B play a critical role during inner ear invagination via modulating histone methylation of the direct target Dlx3.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Rockefeller University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Epigenetics
dc.subject
Inner Ear
dc.subject
Invagination
dc.subject
Dlx3
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-31T17:21:18Z
dc.journal.volume
211
dc.journal.number
4
dc.journal.pagination
815-827
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Uribe, Rosa A.. California Institute of Technology; Estados Unidos
dc.description.fil
Fil: Buzzi, Ailín Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
dc.description.fil
Fil: Bronner, Marianne E.. California Institute of Technology; Estados Unidos
dc.description.fil
Fil: Strobl Mazulla, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
dc.journal.title
Journal of Cell Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1083/jcb.201503071
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/211/4/815
Archivos asociados