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Artículo

Intermediate filament protein expression and sugar moieties in normal canine placenta

Fernández, Patricia Elena; Barbeito, Claudio GustavoIcon ; Portiansky, Enrique LeoIcon ; Gimeno, Eduardo JuanIcon
Fecha de publicación: 01/2000
Editorial: F Hernandez
Revista: Histology and Histopathology
ISSN: 0213-3911
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

In the female dog, the placenta is considered zonal, endotheliochorial and labyrinthic. The distribution of the intermediate filaments as well as the surface glycoproteins in the canine placenta are still unknown. The aim of the present study was to provide this information for further understanding of pathological conditions in the bitch. Samples were obtained from normal uterine horns at the end of gestation. Tissues were routinely fixed and stained. Monoclonal antibodies against cytokeratins, vimentin and desmin were used for immunohistochemical staining. UEA-I, PNA, RCA-I, SBA, DBA, WGA and ConA were used for the lectin histochemical staining. A computer morphometrical analysis was made. Statistical analysis was then accomplished. The results showed the maximun immunohistochemical percentage for vimentin in the supra glandular connective tissue, for pancytokeratin in the spongy zone and for desmin in miometrium. SBA showed the highest staining percentage in the gland cells of the spongy zone, while ConA was the highest in the syncytiotrophoblastic cells and gland cells of the deep glandular zone. The results obtained indicate that the lectin binding pattern is partially different from other animal species. On the contrary, the intermediate filament data coincide with similar observations from other mammals.
Palabras clave: Bitch , Placenta , Lectins , Immunohistochemistry , Glycoconjugates
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/53577
DOI: https://dx.doi.org/10.14670/HH-15.1
URL: http://www.hh.um.es/Abstracts/Vol_15/15_1/15_1_1.htm
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Fernández, Patricia Elena; Barbeito, Claudio Gustavo; Portiansky, Enrique Leo; Gimeno, Eduardo Juan; Intermediate filament protein expression and sugar moieties in normal canine placenta; F Hernandez; Histology and Histopathology; 15; 1-2000; 1-6
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