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dc.contributor.author
Bhakta, Samir A.
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Benavidez, Tomás Enrique
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Garcia, Carlos D.
dc.date.available
2018-07-30T20:48:40Z
dc.date.issued
2014-09-15
dc.identifier.citation
Bhakta, Samir A.; Benavidez, Tomás Enrique; Garcia, Carlos D.; Immobilization of glucose oxidase to nanostructured films of polystyrene-block-poly(2-vinylpyridine); Academic Press Inc Elsevier Science; Journal of Colloid and Interface Science; 430; 15-9-2014; 351-356
dc.identifier.issn
0021-9797
dc.identifier.uri
http://hdl.handle.net/11336/53505
dc.description.abstract
A critical step for the development of biosensors is the immobilization of the biorecognition element to the surface of a substrate. Among other materials that can be used as substrates, block copolymers have the untapped potential to provide significant advantages for the immobilization of proteins. To explore such possibility, this manuscript describes the fabrication and characterization of thin-films of polystyrene-block-poly(2-vinylpyridine) (PS-b-P2VP). These films were then used to investigate the immobilization of glucose oxidase, a model enzyme for the development of biosensors. According to the results presented, the nanoporous films can provide significant increases in surface area of the substrate and the immobilization of larger amounts of active enzyme. The characterization of the substrate-enzyme interface discussed in the manuscript aims to provide critical information about relationship between the surface (material, geometry, and density of pores), the protein structure, and the immobilization conditions (pH, and protein concentration) required to improve the catalytic activity and stability of the enzymes. A maximum normalized activity of 3300±700Um(-2) was achieved for the nanoporous film of PS-b-P2VP.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Adsorption
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Atomic Force Microscopy
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Ellipsometry
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Entrapment
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Glucose Oxidase
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Nanoporous Materials
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Polystyrene-Block-Poly(2-Vinylpyridine)
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Immobilization of glucose oxidase to nanostructured films of polystyrene-block-poly(2-vinylpyridine)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-30T15:24:06Z
dc.journal.volume
430
dc.journal.pagination
351-356
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Bhakta, Samir A.. University Of Texas At San Antonio; Estados Unidos
dc.description.fil
Fil: Benavidez, Tomás Enrique. University Of Texas At San Antonio; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Garcia, Carlos D.. University Of Texas At San Antonio; Estados Unidos
dc.journal.title
Journal of Colloid and Interface Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jcis.2014.05.067
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021979714003853
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