Mostrar el registro sencillo del ítem

dc.contributor.author
Montaner, Alejandro Daniel
dc.contributor.author
Gonzalez, Olga Mabel
dc.contributor.author
Paz, Dante Agustin
dc.contributor.author
Affanni, Jorge Mario
dc.contributor.author
Somoza, Gustavo Manuel
dc.date.available
2018-07-30T16:24:26Z
dc.date.issued
2000-03
dc.identifier.citation
Montaner, Alejandro Daniel; Gonzalez, Olga Mabel; Paz, Dante Agustin; Affanni, Jorge Mario; Somoza, Gustavo Manuel; Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 119; 2; 3-2000; 121-131
dc.identifier.issn
0016-6480
dc.identifier.uri
http://hdl.handle.net/11336/53396
dc.description.abstract
In reptiles as in other vertebrates, multiple forms of gonadotropin-releasing hormone (GnRH) within a single brain have been identified. In this group the following GnRH molecular variants have been characterized either by direct or indirect methods: chicken GnRH I (cGnRH-I), chicken GnRH II (cGnRH-II), salmon GnRH (sGnRH) and several unidentified GnRH-like forms. In the present study GnRH variants were investigated in brain extracts of the lizard Tupinambis teguixin (= T. merinae) by combining high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassays (RIA). Two peaks showing GnRH immunoreactivity with the elution position of synthetic mammalian GnRH (mGnRH) and cGnRH-II were detected. Both peaks were further analyzed with different radioimmunoassay systems specific for mGnRH, cGnRH-I, and cGnRH-II. Pooled fractions corresponding to the first eluting peak showed no cross-reactivity when analyzed with a cGnRH-I specific assay and logit-log displacement curves were not significantly different from those of synthetic mGnRH with homologous RIA systems. The second peak showed immunological characteristics of cGnRH-II when analyzed with a specific antiserum. The first ir-GnRH peak was selected for further RP-HPLC purification showing similar chromatographic behavior as mGnRH synthetic standard. We demonstrated the absence of cGnRH-I in this lizard using well-characterized antisera. (C) 2000 Academic Press.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Brain
dc.subject
Gonadotropin-Releasing Hormone
dc.subject
Reptiles
dc.subject
Ria
dc.subject
Rp-Hplc
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-23T17:54:23Z
dc.journal.volume
119
dc.journal.number
2
dc.journal.pagination
121-131
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Montaner, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Gonzalez, Olga Mabel. Universidad de Buenos Aires. Facultad de Agronomia. Pabellon de Zootécnica; Argentina
dc.description.fil
Fil: Paz, Dante Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Embriología Animal; Argentina
dc.description.fil
Fil: Affanni, Jorge Mario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina
dc.journal.title
General and Comparative Endocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1006/gcen.2000.7488
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0016648000974884


Archivos asociados

Documento no disponible

Comunidades y colecciones

Mostrar el registro sencillo del ítem