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dc.contributor.author
Ternette, Nicola
dc.contributor.author
Yang, Hongbing
dc.contributor.author
Partridge, Thomas
dc.contributor.author
Llano, Anuska
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Cedeño, Samandhy
dc.contributor.author
Fischer, Roman
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Charles, Philip D.
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Dudek, Nadine L.
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Mothe, Beatriz
dc.contributor.author
Crespo, Manuel
dc.contributor.author
Fischer, William M.
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Korber, Bette T. M.
dc.contributor.author
Nielsen, Morten
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Borrow, Persephone
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Purcell, Anthony W.
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Brander, Christian
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Dorrell, Lucy
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Kessler, Benedikt M.
dc.contributor.author
Hanke, Tomáš
dc.date.available
2018-07-27T22:07:50Z
dc.date.issued
2016-01
dc.identifier.citation
Ternette, Nicola; Yang, Hongbing; Partridge, Thomas; Llano, Anuska; Cedeño, Samandhy; et al.; Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells; Wiley VCH Verlag; European Journal of Immunology; 46; 1; 1-2016; 60-69
dc.identifier.issn
0014-2980
dc.identifier.uri
http://hdl.handle.net/11336/53352
dc.description.abstract
Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley VCH Verlag
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Cytotoxic T Cells
dc.subject
Human Immunodeficiency Virus Type I
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Human Leukocyte Antigen
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Immunopeptidome
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Mass Spectrometry
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Salud Ocupacional
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-19T16:51:35Z
dc.journal.volume
46
dc.journal.number
1
dc.journal.pagination
60-69
dc.journal.pais
Alemania
dc.journal.ciudad
Weinheim
dc.description.fil
Fil: Ternette, Nicola. University of Oxford; Reino Unido
dc.description.fil
Fil: Yang, Hongbing. University of Oxford; Reino Unido
dc.description.fil
Fil: Partridge, Thomas. University of Oxford; Reino Unido
dc.description.fil
Fil: Llano, Anuska. Universitat Autònoma de Barcelona; España
dc.description.fil
Fil: Cedeño, Samandhy. Universitat Autònoma de Barcelona; España
dc.description.fil
Fil: Fischer, Roman. University of Oxford; Reino Unido
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Fil: Charles, Philip D.. University of Oxford; Reino Unido
dc.description.fil
Fil: Dudek, Nadine L.. Monash University; Australia
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Fil: Mothe, Beatriz. Hospital Universitari Germans Trias I Pujol; . Universitat Autònoma de Barcelona; España. Hospital Germans Trias i Pujol; España
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Fil: Crespo, Manuel. Universitat Central de Catalunya; España. Hospital de la Vall d'Hebrón. HIV Unit; España
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Fil: Fischer, William M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
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Fil: Korber, Bette T. M.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
dc.description.fil
Fil: Nielsen, Morten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Technical University of Denmark; Dinamarca
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Fil: Borrow, Persephone. University of Oxford; Reino Unido
dc.description.fil
Fil: Purcell, Anthony W.. Monash University; Australia
dc.description.fil
Fil: Brander, Christian. Universitat Autònoma de Barcelona; España. Institució Catalana de Recerca i Estudis Avancats; España. Universitat Central de Catalunya; España
dc.description.fil
Fil: Dorrell, Lucy. University of Oxford; Reino Unido
dc.description.fil
Fil: Kessler, Benedikt M.. University of Oxford; Reino Unido
dc.description.fil
Fil: Hanke, Tomáš. University of Oxford; Reino Unido
dc.journal.title
European Journal of Immunology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1002/eji.201545890
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.201545890
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737398/
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