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dc.contributor.author Panero, Julieta
dc.contributor.author Arbelbide, Jorge
dc.contributor.author Fantl, Dorotea Beatriz
dc.contributor.author Garcia Rivello, Hernan Jorge
dc.contributor.author Kohan, Dana
dc.contributor.author Slavutsky, Irma Rosa
dc.date.available 2018-07-27T15:07:28Z
dc.date.issued 2010-11
dc.identifier.citation Panero, Julieta; Arbelbide, Jorge; Fantl, Dorotea Beatriz; Garcia Rivello, Hernan Jorge; Kohan, Dana; et al.; Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma; Feinstein Inst Med Res; Molecular Medicine; 16; 11-12; 11-2010; 471-478
dc.identifier.issn 1528-3658
dc.identifier.uri http://hdl.handle.net/11336/53289
dc.description.abstract In this study, we explored changes in the expression of the telomere maintenance genes, TRF1, TRF2 and TANK1 in patients with monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM). Results were correlated with human telomerase reverse transcriptase (hTERT) expression, telomere length (TL) and clinicopathological characteristics. Bone marrow (BM) samples from 132 patients, 64 with MGUS and 68 with MM, were studied. Real-time quantitative reverse transcription-polymerase chain reaction was used to quantify gene expression. TL was evaluated by terminal restriction fragment length analysis. MGUS patients showed increased TRF1 levels (P = 0.006) and lower expression of TRF2 (P = 0.005) and TANK1 (P = 0.003) compared with MM patients. For hTERT analysis, patients were divided into three groups by use of receiver operating characteristics: low (group I [GI]), intermediate (group II [GII]) and high (group III [GIII]) expression. We observed increasing expression of TRF2 and TANK1 from GI to GIII in MGUS and MM, with differences for both genes in MM (P < 0.01) and for TRF2 in MGUS (P < 0.01). GIII patients with the highest telomerase expression had the shortest TL. In both entities, a positive association between TRF2-TANK1, TRF2-hTERT and TANK1-hTERT (P ≤ 0.01) was observed. In MM, the percentage of BM infiltration and Ki-67 index were positively associated with TRF2, TANK1 and hTERT expression (P ≤ 0.03) and negatively with TL (P = 0.02), whereas lactate dehydrogenase was significantly correlated with TRF2 mRNA (P = 0.008). Our findings provide the first evidence of a modification in the expression of telomeric proteins in plasma cell disorders, and suggest that mechanisms other than telomerase activation are involved in TL maintenance in these pathologies. © 2010 The Feinstein Institute for Medical Research.
dc.format application/pdf
dc.language.iso eng
dc.publisher Feinstein Inst Med Res
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject Myeloma
dc.subject MGUS
dc.subject Telomere
dc.subject.classification Inmunología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-07-23T18:28:57Z
dc.journal.volume 16
dc.journal.number 11-12
dc.journal.pagination 471-478
dc.journal.pais Estados Unidos
dc.journal.ciudad Manhasset
dc.description.fil Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
dc.description.fil Fil: Arbelbide, Jorge. Hospital Italiano; Argentina
dc.description.fil Fil: Fantl, Dorotea Beatriz. Hospital Italiano; Argentina
dc.description.fil Fil: Garcia Rivello, Hernan Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
dc.description.fil Fil: Kohan, Dana. Hospital Italiano; Argentina
dc.description.fil Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
dc.journal.title Molecular Medicine
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.2119/molmed.2010.00057
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://static.smallworldlabs.com/molmedcommunity/content/pdfstore/10_57_Panero.pdf
dc.conicet.fuente unificacion


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)