Mostrar el registro sencillo del ítem
dc.contributor.author
Martínez Júlvez, Marta
dc.contributor.author
Goñi Rasia, Guillermina Marcela
dc.contributor.author
Pérez Amigot, Daniel
dc.contributor.author
Laplaza, Rubén
dc.contributor.author
Ionescu, Irina Alexandra
dc.contributor.author
Petrocelli, Silvana
dc.contributor.author
Tondo, Maria Laura
dc.contributor.author
Sancho, Javier
dc.contributor.author
Orellano, Elena Graciela
dc.contributor.author
Medina, Milagros
dc.date.available
2018-07-26T19:54:23Z
dc.date.issued
2017-12
dc.identifier.citation
Martínez Júlvez, Marta; Goñi Rasia, Guillermina Marcela; Pérez Amigot, Daniel; Laplaza, Rubén; Ionescu, Irina Alexandra; et al.; Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria; Molecular Diversity Preservation International; Molecules; 23; 1; 12-2017; 1-15
dc.identifier.issn
1420-3049
dc.identifier.uri
http://hdl.handle.net/11336/53227
dc.description.abstract
Ferredoxin-NADP(H) reductases (FNRs) deliver NADPH or low potential one-electron donors to redox-based metabolism in plastids and bacteria. Xanthomonas citri subsp. citri (Xcc) is a Gram-negative bacterium responsible for citrus canker disease that affects commercial citrus crops worldwide. The Xcc fpr gene encodes a bacterial type FNR (XccFPR) that contributes to the bacterial response to oxidative stress conditions, usually found during plant colonization. Therefore, XccFPR is relevant for the pathogen survival and its inhibition might represent a strategy to treat citrus canker. Because of mechanistic and structural differences from plastidic FNRs, XccFPR is also a potential antibacterial target. We have optimized an activity-based high-throughput screening (HTS) assay that identifies XccFPR inhibitors. We selected 43 hits from a chemical library and narrowed them down to the four most promising inhibitors. The antimicrobial effect of these compounds was evaluated on Xcc cultures, finding one with antimicrobial properties. Based on the functional groups of this compound and their geometric arrangement, we identified another three XccFPR inhibitors. Inhibition mechanisms and constants were determined for these four XccFPR inhibitors. Their specificity was also evaluated by studying their effect on the plastidic Anabaena PCC 7119 FNR, finding differences that can become interesting tools to discover Xcc antimicrobials.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Molecular Diversity Preservation International
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Activity-Based High-Throughput Screening
dc.subject
Enzyme Inhibitors
dc.subject
Ferredoxin-Nadp(H) Reductase
dc.subject
Xanthomonas Citri Subsp. Citri
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Identification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-28T14:14:34Z
dc.journal.volume
23
dc.journal.number
1
dc.journal.pagination
1-15
dc.journal.pais
Suiza
dc.journal.ciudad
Basel
dc.description.fil
Fil: Martínez Júlvez, Marta. Universidad de Zaragoza; España
dc.description.fil
Fil: Goñi Rasia, Guillermina Marcela. Universidad de Zaragoza; España
dc.description.fil
Fil: Pérez Amigot, Daniel. Universidad de Zaragoza; España
dc.description.fil
Fil: Laplaza, Rubén. Universidad de Zaragoza; España. Universidad de Santiago de Compostela; España
dc.description.fil
Fil: Ionescu, Irina Alexandra. Universidad de Zaragoza; España
dc.description.fil
Fil: Petrocelli, Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Tondo, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Sancho, Javier. Universidad de Zaragoza; España
dc.description.fil
Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Medina, Milagros. Universidad de Zaragoza; España
dc.journal.title
Molecules
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.3390/molecules23010029
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1420-3049/23/1/29
Archivos asociados