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dc.contributor.author
Vera Pingitore, Esteban
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Hebert, Elvira Maria
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Sesma, Fernando Juan Manuel
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Nader, Maria Elena Fatima
dc.date.available
2018-07-26T19:05:51Z
dc.date.issued
2009-03
dc.identifier.citation
Vera Pingitore, Esteban; Hebert, Elvira Maria; Sesma, Fernando Juan Manuel; Nader, Maria Elena Fatima; Influence of vitamins and osmolites on growth and bacteriocin production by Lactobacillus salivarius CRL 1328 in a chemically defined medium; National Research Council Canada-NRC Research Press; Canadian Journal Of Microbiology; 55; 3; 3-2009; 304-310
dc.identifier.issn
0008-4166
dc.identifier.uri
http://hdl.handle.net/11336/53194
dc.description.abstract
The aim of this study was to analyze the influence of vitamins, glycerol and salts on the growth and bacteriocin production by Lactobacillus salivarius CRL 1328, a human vagina isolate, by using a chemically defined medium, in order to define the optimal conditions for salivaricin production. The single omission of d-biotin, thiamine, p-aminobenzoic acid, folic acid or cyanocobalamin did not affect the bacterial growth, while the removal of nicotinic acid, riboflavin and pyridoxal produced a decrease of about 30% in the growth rate. Maximum salivaricin activity was observed after the addition of 5 or 10 g/l of NaCl. On the basis of the nutritional requirements and the levels of salivaricin production, a new optimized and simplified defined medium (SDM-NaCl) for L. salivarius CRL 1328 bacteriocin production was formulated. The kinetics of salivaricin production in SDM-NaCl and in the complex media LAPTg revealed that bacteriocin production was growth linked. A combination of Tricine-SDS-PAGE, Lumitein protein gel staining and a bioassay for antibacterial activity indicated that the molecular weight of salivaricin CRL 1328 is about 4.5 kDa. The partially purified bacteriocin, obtained from SDM-NaCl after concentration, allowed designing a relatively simple method for the recovery of a biologically active protein.
dc.description.abstract
Le but de cette étude était d’analyser l’influence des vitamines, du glycérol et des sels sur la croissance et la production de bactériocines par Lactobacillus salivarius CRL 1328, un isolat vaginal humain, en utilisant un milieu de culture chimique défini, afin de déterminer les conditions optimales de production de salivaricine. L’omission simple de Dbiotine, de thiamine, d’acide p-aminobenzoı¨que, d’acide folique ou de cyanocobalamine n’affectait pas la croissance bacté- rienne, alors que le retrait d’acide nicotinique, de riboflavine et de pyridoxal diminuait le taux de croissance d’environ 30 %. L’activité maximale liée a` la salivaricine était observée apre`s l’ajout de 5 ou 10 g/L de NaCl. Sur la base des besoins nutritionnels et des niveaux de production de salivaricine, un nouveau milieu défini optimal et simplifié (SDM-NaCl) a été formulé pour la production de salivaricine par L. Salivarius CRL 1328. Les cine´tiques de production de salivaricine dans le milieu SDM-NaCl et dans le milieu complexe LAPTg ont révélé que la production de la bactériocine était liée a` la
croissance. La combinaison d’une électrophore`se sur gel de polyacrylamide Tricine–SDS, d’une coloration protéique sur gel par la Lumitein et d’un essai biologique de l’activité antibactérienne a indiqué que le poids moléculaire de la salivaricine de CRL 1328 est d’environ 4,5 kDa. La bactériocine partiellement purifieé du milieu SDM–NaCl concentré a permis d’é laborer une me´thode relativement simple de récupération de la protéine biologiquement active.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
National Research Council Canada-NRC Research Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bacteriocin
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Lactic Acid Bacteria
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Lactobacillus
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Salivaricin
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Vagina
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Influence of vitamins and osmolites on growth and bacteriocin production by Lactobacillus salivarius CRL 1328 in a chemically defined medium
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-16T14:29:21Z
dc.identifier.eissn
1480-3275
dc.journal.volume
55
dc.journal.number
3
dc.journal.pagination
304-310
dc.journal.pais
Canadá
dc.journal.ciudad
Otawa
dc.description.fil
Fil: Vera Pingitore, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.journal.title
Canadian Journal Of Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1139/w08-092
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/W08-092#.W1obbNVKiUk
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