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dc.contributor.author
Loureiro, Dana Belen  
dc.contributor.author
Romanini, Diana  
dc.contributor.author
Tubio, Gisela  
dc.date.available
2018-07-24T15:54:28Z  
dc.date.issued
2016-01  
dc.identifier.citation
Loureiro, Dana Belen; Romanini, Diana; Tubio, Gisela; Structural and functional analysis of Aspergillus niger xylanase to be employed in polyethylenglycol/salt aqueous two-phase extraction; Elsevier; Biocatalysis and Agricultural Biotechnology; 5; 1-2016; 204-210  
dc.identifier.issn
1878-8181  
dc.identifier.uri
http://hdl.handle.net/11336/52962  
dc.description.abstract
The structure and enzymatic activity of Aspergillus niger xylanase were evaluated in different media to establish an appropriate protocol for the extraction of the enzyme in polymer/salt aqueous two-phase systems. Different factors were studied: the concentration and molecular weight (1000, 2000, 4600 and 8000) of polyethyleneglycol, the concentration and type of salt (sodium citrate and potassium phosphate) and pH, time and temperature. Xylanase was stable for 5 h at pH between 2.7 and 9.0 and at temperatures up to 50 °C. Fluorescence spectroscopy and circular dichroism experiments showed that neither the secondary/tertiary structure of the enzyme nor its catalytic activity were significantly altered in the presence of either salt or PEG. Xylanase partitioned into the PEG-rich phase driven by the excluded volume effect. Partitioning was more favorable to the polymer phase in the PEG1000/NaCit system, where Kp was 12 times higher than in the others aqueous two-phase systems. These results demonstrate the potential application of the PEG1000/NaCit system as a first step for the extraction of Aspergillus niger xylanase.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Aqueous Two-Phase Extraction  
dc.subject
Aspergillus Niger  
dc.subject
Polyethyleneglycol  
dc.subject
Xylanase  
dc.subject.classification
Bioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental  
dc.subject.classification
Biotecnología del Medio Ambiente  
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Structural and functional analysis of Aspergillus niger xylanase to be employed in polyethylenglycol/salt aqueous two-phase extraction  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-07-24T14:47:39Z  
dc.journal.volume
5  
dc.journal.pagination
204-210  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Loureiro, Dana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina  
dc.description.fil
Fil: Romanini, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina  
dc.description.fil
Fil: Tubio, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina  
dc.journal.title
Biocatalysis and Agricultural Biotechnology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.bcab.2015.12.008  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1878818115300281