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dc.contributor.author
Irigoyen, María Belén
dc.contributor.author
Primiani, L.
dc.contributor.author
Felippo, Marta Elena
dc.contributor.author
Candela, M.
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Perez Bianco, R.
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De Bracco, M. M. DE E.
dc.contributor.author
Galassi, Nora Virginia
dc.date.available
2018-07-23T20:55:40Z
dc.date.issued
2011-03
dc.identifier.citation
Irigoyen, María Belén; Primiani, L.; Felippo, Marta Elena; Candela, M.; Perez Bianco, R.; et al.; A flow cytometry evaluation of anti-FVIII antibodies: Correlation with ELISA and Bethesda assay; Wiley Blackwell Publishing, Inc; Haemophilia The Official Journal Of The World Federation Of Hemophilia; 17; 2; 3-2011; 267-274
dc.identifier.issn
1351-8216
dc.identifier.uri
http://hdl.handle.net/11336/52932
dc.description.abstract
In this study, we describe a flow cytometry (FC) system for detecting antibodies to factor VIII (FVIII) and compare its results with those of enzyme-linked immunosorbent assay (ELISA) that detects both inhibitory (I-Ab) and non-inhibitory (NI-Ab) antibodies and the Nijmegen modification of the Bethesda method, detecting I-Ab. FC was set up in our laboratory. Recombinant FVIII (rFVIII) was coupled to microspheres (FVIII-m) and reacted with different plasma dilutions. Microspheres without rFVIII were used as control (control-m). Captured anti-FVIII antibodies were detected using anti-human IgG. Plasma samples from the following patients with severe haemophilia A (SHA) patients were evaluated: 17 P (patients without I-Ab, <0.5BUmL-1); 13 PI (patients with I-Ab, 1.1-8200BUmL-1). Of these 13, two PI were referred during immune tolerance induction (ITI), and plasmas from 12 healthy donors (HD) were evaluated. Semiquantitative results were given as an index (the highest mean fluorescence intensity ratio between FVIII-m and control-m multiplied by the inverse of the corresponding plasma dilution). Both plasma and serum were suitable for the test. FC agreed with the Bethesda method (r=0.8; P=0.0001). FC and ELISA had 80% of coincidence. Four of 17 patients (23.5%) had NI-Ab by FC, and two of them developed high levels of I-Ab later on. This test provides a useful alternative for measuring FVIII antibodies supplementing Bethesda assay. FC is fast and easy to perform. No more than 200μL of plasma or serum is required especially making it useful for paediatric patients. © 2010 Blackwell Publishing Ltd.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Flow Cytometry
dc.subject
Fviii Antibodies
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Haemophilia A
dc.subject
Microspheres
dc.subject.classification
Medicina Critica y de Emergencia
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Medicina Clínica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
A flow cytometry evaluation of anti-FVIII antibodies: Correlation with ELISA and Bethesda assay
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-23T18:28:35Z
dc.journal.volume
17
dc.journal.number
2
dc.journal.pagination
267-274
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Irigoyen, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil
Fil: Primiani, L.. Fundación Argentina de Hemofilia; Argentina
dc.description.fil
Fil: Felippo, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil
Fil: Candela, M.. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil
Fil: Perez Bianco, R.. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil
Fil: De Bracco, M. M. DE E.. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil
Fil: Galassi, Nora Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.journal.title
Haemophilia The Official Journal Of The World Federation Of Hemophilia
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1111/j.1365-2516.2010.02406.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2516.2010.02406.x


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