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dc.contributor.author
García, Paula
dc.contributor.author
Ramallo, Ivana Ayelen
dc.contributor.author
Furlan, Ricardo Luis Eugenio
dc.date.available
2018-07-23T19:43:19Z
dc.date.issued
2017-03
dc.identifier.citation
García, Paula; Ramallo, Ivana Ayelen; Furlan, Ricardo Luis Eugenio; Reverse Phase Compatible TLC-Bioautography for Detection of Tyrosinase Inhibitors; John Wiley & Sons Ltd; Phytochemical Analysis; 28; 2; 3-2017; 101-105
dc.identifier.issn
0958-0344
dc.identifier.uri
http://hdl.handle.net/11336/52887
dc.description.abstract
Introduction: Reverse phase chromatography and bioautographic assays are key tools for natural product bioguided isolation; however, their direct coupling has not been fully achieved. Objectives: To develop a bioautographic assay to detect tyrosinase inhibitors present in complex matrices sorbed on reverse phase (RP) TLC-plates that can be used for bioguided isolation of bioactive compounds. Methods: Enzyme gel entrapment with an amphiphilic copolymer was used for assay development. The gel turns into a brown “skin like” colour due to tyrosinase catalysed oxidation of l-tyrosine. The inhibitors are visualised as clear spots against a brown coloured background. Results: The assay was able to localise cinnamaldehyde in Cinnamomum cassia essential oil, as its main constituent with known tyrosinase inhibition properties. The assay allowed the detection of 0.03% (w/w) of kojic acid co-spotted with a methanolic extract of Sphaeralcea bonariensis and chromatographed on RP-TLC. Conclusion: The developed assay is able to detect, with high sensitivity, tyrosinase inhibitors present in complex matrices that were chromatographed in RP-TLC. Results can be easily read by colour change, inhibitors appear as clear spots in a darker background. Copyright © 2016 John Wiley & Sons, Ltd.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Dermatological Disorders
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Herbal Drugs
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Poloxamers
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Tlc-Bioautography
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Tyrosinase Inhibition
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Otras Ciencias Químicas
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Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Reverse Phase Compatible TLC-Bioautography for Detection of Tyrosinase Inhibitors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-23T12:55:27Z
dc.journal.volume
28
dc.journal.number
2
dc.journal.pagination
101-105
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: García, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario. Universidad Nacional de Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario; Argentina
dc.description.fil
Fil: Ramallo, Ivana Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario. Universidad Nacional de Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario; Argentina
dc.description.fil
Fil: Furlan, Ricardo Luis Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario. Universidad Nacional de Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario; Argentina
dc.journal.title
Phytochemical Analysis
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1002/pca.2655
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.2655


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