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dc.contributor.author Falcone Ferreyra, María Lorena
dc.contributor.author Casadevall, Romina
dc.contributor.author D'andrea, Lucio Jesus
dc.contributor.author Abdelgawad, Hamada
dc.contributor.author Beemster, Gerrit T.S.
dc.contributor.author Casati, Paula
dc.date.available 2018-07-23T17:23:07Z
dc.date.issued 2016-04
dc.identifier.citation Falcone Ferreyra, María Lorena; Casadevall, Romina; D'andrea, Lucio Jesus; Abdelgawad, Hamada; Beemster, Gerrit T.S.; et al.; AtPDCD5 plays a role in programmed cell death after UV-B exposure in arabidopsis; American Society of Plant Biologist; Plant Physiology; 170; 4; 4-2016; 2444-2460
dc.identifier.issn 0032-0889
dc.identifier.uri http://hdl.handle.net/11336/52827
dc.description.abstract DNA damage responses have evolved to sense and react to DNA damage; the induction of DNA repair mechanisms can lead to genomic restoration or, if the damaged DNA cannot be adequately repaired, to the execution of a cell death program. In this work, we investigated the role of an Arabidopsis (Arabidopsis thaliana) protein, AtPDCD5, which is highly similar to the human PDCD5 protein; it is induced by ultraviolet (UV)-B radiation and participates in programmed cell death in the UV-B DNA damage response. Transgenic plants expressing AtPDCD5 fused to GREEN FLUORESCENT PROTEIN indicate that AtPDCD5 is localized both in the nucleus and the cytosol. By use of pdcd5 mutants, we here demonstrate that these plants have an altered antioxidant metabolism and accumulate higher levels of DNA damage after UV-B exposure, similar to levels in ham1ham2 RNA interference transgenic lines with decreased expression of acetyltransferases from the MYST family. By coimmunoprecipitation and pull-down assays, we provide evidence that AtPDCD5 interacts with HAM proteins, suggesting that both proteins participate in the same pathway of DNA damage responses. Plants overexpressing AtPDCD5 show less DNA damage but more cell death in root tips upon UV-B exposure. Finally, we here show that AtPDCD5 also participates in age-induced programmed cell death. Together, the data presented here demonstrate that AtPDCD5 plays an important role during DNA damage responses induced by UV-B radiation in Arabidopsis and also participates in programmed cell death programs.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Society of Plant Biologist
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject PDCD5
dc.subject UV-B radiation
dc.subject Senescence
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title AtPDCD5 plays a role in programmed cell death after UV-B exposure in arabidopsis
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-07-18T20:42:40Z
dc.journal.volume 170
dc.journal.number 4
dc.journal.pagination 2444-2460
dc.journal.pais Estados Unidos
dc.journal.ciudad Rockville
dc.description.fil Fil: Falcone Ferreyra, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
dc.description.fil Fil: Casadevall, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
dc.description.fil Fil: D'andrea, Lucio Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
dc.description.fil Fil: Abdelgawad, Hamada. Universiteit Antwerp; Bélgica. University of Beni-Suef; Egipto
dc.description.fil Fil: Beemster, Gerrit T.S.. Universiteit Antwerp; Bélgica
dc.description.fil Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
dc.journal.title Plant Physiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1104/pp.16.00033
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/170/4/2444
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)