Artículo
Design of a Simple Slow Cooling Device for Cryopreservation of Small Biological Samples
Juan de Paz, Leonardo; Robert, María Celeste
; Graf, Daniel Adolfo; Guibert, Edgardo Elvio
; Rodriguez, Joaquin Valentin
Fecha de publicación:
11/2015
Editorial:
Cryo Letters
Revista:
Cryo-letters
ISSN:
0143-2044
e-ISSN:
1742-0644
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
BACKGROUND: Slow cooling is a cryopreservation methodology where samples are cooled to its storage temperature at controlled cooling rates. OBJECTIVE: Design, construction and evaluation of a simple and low cost device for slow cooling of small biological samples. MATERIALS AND METHODS: The device was constructed based on Pye’s freezer idea. A Dewar flask filled with liquid nitrogen was used as heat sink and a methanol bath containing the sample was cooled at constant rates using copper bars as heat conductor. RESULTS: Sample temperature may be lowered at controlled cooling rate (ranging from 0.4ºC/min to 6.0ºC/min) down to ~-60ºC, where it could be conserved at lower temperatures. An example involving the cryopreservation of Neuro-2A cell line showed a marked influence of cooling rate over post preservation cell viability with optimal values between 2.6 and 4.6°C/min. CONCLUSION: The cooling device proved to be a valuable alternative to more expensive systems allowing the assessment of different cooling rates to evaluate the optimal condition for cryopreservation of such samples.
Palabras clave:
Slow Cooling Device
,
Cooling Rate
,
Cryopreservation
,
Neural Cells
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Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Citación
Juan de Paz, Leonardo; Robert, María Celeste; Graf, Daniel Adolfo; Guibert, Edgardo Elvio; Rodriguez, Joaquin Valentin; Design of a Simple Slow Cooling Device for Cryopreservation of Small Biological Samples; Cryo Letters; Cryo-letters; 36; 6; 11-2015; 363-371
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