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dc.contributor.author
De la Guardia, Rafael Díaz
dc.contributor.author
Catalina, Purificación
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Panero, Julieta
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Elosua, Carolina
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Pulgarin, Andrés
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López, María Belén
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Ayllón, Verónica
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Ligero, Gertrudis
dc.contributor.author
Slavutsky, Irma Rosa
dc.contributor.author
Leone, Paola E.
dc.date.available
2018-07-18T20:10:10Z
dc.date.issued
2012-12
dc.identifier.citation
De la Guardia, Rafael Díaz; Catalina, Purificación; Panero, Julieta; Elosua, Carolina; Pulgarin, Andrés; et al.; Expression profile of telomere-associated genes in multiple myeloma; Wiley Blackwell Publishing, Inc; Journal Of Cellular And Molecular Medicine (print); 16; 12; 12-2012; 3009-3021
dc.identifier.issn
1582-1838
dc.identifier.uri
http://hdl.handle.net/11336/52611
dc.description.abstract
To further contribute to the understanding of multiple myeloma, we have focused our research interests on the mechanisms by which tumour plasma cells have a higher survival rate than normal plasma cells. In this article, we study the expression profile of genes involved in the regulation and protection of telomere length, telomerase activity and apoptosis in samples from patients with monoclonal gammopathy of undetermined significance, smouldering multiple myeloma, multiple myeloma (MM) and plasma cell leukaemia (PCL), as well as several human myeloma cell lines (HMCLs). Using conventional cytogenetic and fluorescence in situ hybridization studies, we identified a high number of telomeric associations (TAs). Moreover, telomere length measurements by terminal restriction fragment (TRF) assay showed a shorter mean TRF peak value, with a consistent correlation with the number of TAs. Using gene expression arrays and quantitative PCR we identified the hTERT gene together with 16 other genes directly involved in telomere length maintenance: HSPA9, KRAS, RB1, members of the Small nucleolar ribonucleoproteins family, A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins, and 14-3-3 family. The expression levels of these genes were even higher than those in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), which have unlimited proliferation capacity. In conclusion, the gene signature suggests that MM tumour cells are able to maintain stable short telomere lengths without exceeding the short critical length, allowing cell divisions to continue. We propose that this could be a mechanism contributing to MM tumour cells expansion in the bone marrow (BM). © 2012 The Authors Journal of Cellular and Molecular Medicine © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Apoptosis
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Cell Survival
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Chromosomal Instability
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Multiple Myeloma
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Telomere Length Maintenance
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Telomeric Associations
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Genética Humana
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Expression profile of telomere-associated genes in multiple myeloma
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-17T20:49:13Z
dc.journal.volume
16
dc.journal.number
12
dc.journal.pagination
3009-3021
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: De la Guardia, Rafael Díaz. Universidad de Granada; España
dc.description.fil
Fil: Catalina, Purificación. Universidad de Granada; España
dc.description.fil
Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
dc.description.fil
Fil: Elosua, Carolina. Universidad de Granada; España
dc.description.fil
Fil: Pulgarin, Andrés. Universidad de Granada; España
dc.description.fil
Fil: López, María Belén. Universidad de Granada; España
dc.description.fil
Fil: Ayllón, Verónica. Universidad de Granada; España
dc.description.fil
Fil: Ligero, Gertrudis. Universidad de Granada; España
dc.description.fil
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
dc.description.fil
Fil: Leone, Paola E.. Universidad de Granada; España
dc.journal.title
Journal Of Cellular And Molecular Medicine (print)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1111/j.1582-4934.2012.01628.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1582-4934.2012.01628.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393729/
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