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dc.contributor.author
Siena, Lorena Adelina
dc.contributor.author
Ortiz, Juan Pablo Amelio
dc.contributor.author
Calderini, Ornella
dc.contributor.author
Paolocci, Francesco
dc.contributor.author
Cáceres, Maria E.
dc.contributor.author
Kaushal, Pankaj
dc.contributor.author
Grisan, Simone
dc.contributor.author
Pessino, Silvina Claudia
dc.contributor.author
Pupilli, Fulvio
dc.date.available
2018-07-17T19:49:35Z
dc.date.issued
2016-03
dc.identifier.citation
Siena, Lorena Adelina; Ortiz, Juan Pablo Amelio; Calderini, Ornella; Paolocci, Francesco; Cáceres, Maria E.; et al.; An apomixis-linked ORC3-like pseudogene is associated with silencing of its functional homolog in apomictic Paspalum simplex; Oxford University Press; Journal of Experimental Botany; 67; 6; 3-2016; 1965-1978
dc.identifier.issn
0022-0957
dc.identifier.uri
http://hdl.handle.net/11336/52508
dc.description.abstract
Apomixis in plants consists of asexual reproduction by seeds. Here we characterized at structural and functional levels an apomixis-linked sequence of Paspalum simplex homologous to subunit 3 of the ORIGIN RECOGNITION COMPLEX (ORC3). ORC is a multiprotein complex which controls DNA replication and cell differentiation in eukaryotes. Three PsORC3 copies were identified, each one characterized by a specific expression profile. Of these, PsORC3a, specific for apomictic genotypes, is a pseudogene that was poorly and constitutively expressed in all developmental stages of apomictic flowers, whereas PsORC3b, the putative functional gene in sexual flowers, showed a precise time-related regulation. Sense transcripts of PsORC3 were expressed in the female cell lineage of both apomictic and sexual reproductive phenotypes, and in aposporous initials. Although strong expression was detected in sexual early endosperm, no expression was present in the apomictic endosperm. Antisense PsORC3 transcripts were revealed exclusively in apomictic germ cell lineages. Defective orc3 mutants of rice and Arabidopsis showed normal female gametophytes although the embryo and endosperm were arrested at early phases of development. We hypothesize that PsORC3a is associated with the down-regulation of its functional homolog and with the development of apomictic endosperm which deviates from the canonical 2(maternal):1(paternal) genome ratio.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Apomixis
dc.subject
Gene Silencing
dc.subject
Origin Recognition Complex
dc.subject
Paspalum
dc.subject
Seed Development
dc.subject.classification
Agricultura
dc.subject.classification
Agricultura, Silvicultura y Pesca
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
An apomixis-linked ORC3-like pseudogene is associated with silencing of its functional homolog in apomictic Paspalum simplex
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-17T14:07:16Z
dc.journal.volume
67
dc.journal.number
6
dc.journal.pagination
1965-1978
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Siena, Lorena Adelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
dc.description.fil
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
dc.description.fil
Fil: Calderini, Ornella. Istituto di Bioscienze e Biorisorse; Italia
dc.description.fil
Fil: Paolocci, Francesco. Istituto di Bioscienze e Biorisorse; Italia
dc.description.fil
Fil: Cáceres, Maria E.. Istituto di Bioscienze e Biorisorse; Italia
dc.description.fil
Fil: Kaushal, Pankaj. Istituto di Bioscienze e Biorisorse; Italia
dc.description.fil
Fil: Grisan, Simone. Istituto di Bioscienze e Biorisorse; Italia
dc.description.fil
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
dc.description.fil
Fil: Pupilli, Fulvio. Istituto di Bioscienze e Biorisorse; Italia
dc.journal.title
Journal of Experimental Botany
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/jxb/erw018
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/67/6/1965/2885164


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