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dc.contributor.author
Sesto Cabral, María Eugenia
dc.contributor.author
Sigstad, Emma Elizabeth
dc.date.available
2018-07-17T18:40:19Z
dc.date.issued
2011-04
dc.identifier.citation
Sesto Cabral, María Eugenia; Sigstad, Emma Elizabeth; A new approach to determine soil microbial biomass by calorimetry; Springer; Journal of Thermal Analysis and Calorimetry; 104; 1; 4-2011; 23-29
dc.identifier.issn
1388-6150
dc.identifier.uri
http://hdl.handle.net/11336/52481
dc.description.abstract
A method to determine soil microbial biomass (SMB) by isothermal calorimetry is reported. Soil samples ranging in pH from 6.2 to 9.4 and different textures were used to develop the method. Soil at 60% of its field capacity humidity was amended with a previously determined amount of glucose as to give the maximal response of CO2 evolution. Then, an aliquot was weighed in the calorimeter ampoule and specific thermal power (p)-time (t) curves were obtained at 25 °C. After 1-2 h, a vial containing a 0.5 M NaOH solution was introduced to determine the specific thermal power due to CO 2 evolution, pCO2 during 1-2 h. Then, the vial was removed and the experiment continued for 1-2 h. Specific thermal power due to CO 2 evolution was converted to rate (CO2-C/mm3 g-1 h-1) by using the heat of reaction of CO2 with NaOH and the molar volume. This value was further converted into SMB/μg g-1 by using a conversion factor of 32.4. A guide to perform the calculations is given. Values of log SMB were linearly related with values of log p giving a similar relation to a previously reported where SMB was determined by conventional methods.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Calorimetry
dc.subject
Microbial Biomass
dc.subject
Soil
dc.subject.classification
Otras Ciencias Químicas
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
A new approach to determine soil microbial biomass by calorimetry
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-11T15:23:19Z
dc.identifier.eissn
1572-8943
dc.journal.volume
104
dc.journal.number
1
dc.journal.pagination
23-29
dc.journal.pais
Alemania
dc.journal.ciudad
Berlín
dc.description.fil
Fil: Sesto Cabral, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
dc.description.fil
Fil: Sigstad, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
dc.journal.title
Journal of Thermal Analysis and Calorimetry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.springerlink.com/content/q47n2x3270561m55/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s10973-010-1091-3
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