Artículo
G-quadruplexes as novel cis-elements controlling transcription during embryonic development
David, Aldana Paula
; Margarit, Ezequiel
; Domizi, Pablo Daniel
; Banchio, Claudia Elena
; Armas, Pablo
; Calcaterra, Nora Beatriz
Fecha de publicación:
05/2016
Editorial:
Oxford University Press
Revista:
Nucleic Acids Research
ISSN:
1362-4962
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
G-quadruplexes are dynamic structures folded in G-rich single-stranded DNA regions. These structures have been recognized as a potential nucleic acid based mechanism for regulating multiple cellular processes such as replication, transcription and genomic maintenance. So far, their transcriptional role in vivo during vertebrate embryonic development has not yet been addressed. Here, we performed an in silico search to find conserved putative G-quadruplex sequences (PQSs) within proximal promoter regions of human, mouse and zebrafish developmental genes. Among the PQSs able to fold in vitro as G-quadruplex, those present in nog3, col2a1 and fzd5 promoters were selected for further studies. In cellulo studies revealed that the selected G-quadruplexes affected the transcription of luciferase controlled by the SV40 nonrelated promoter. G-quadruplex disruption in vivo by microinjection in zebrafish embryos of either small ligands or DNA oligonucleotides complementary to the selected PQSs resulted in lower transcription of the targeted genes. Moreover, zebrafish embryos and larvae phenotypes caused by the presence of complementary oligonucleotides fully resembled those ones reported for nog3, col2a1 and fzd5 morphants. To our knowledge, this is the first work revealing in vivo the role of conserved G-quadruplexes in the embryonic development, one of the most regulated processes of the vertebrates biology.
Palabras clave:
G-Cuadruples
,
Regulacion Trasncripcional
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Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
David, Aldana Paula; Margarit, Ezequiel; Domizi, Pablo Daniel; Banchio, Claudia Elena; Armas, Pablo; et al.; G-quadruplexes as novel cis-elements controlling transcription during embryonic development; Oxford University Press; Nucleic Acids Research; 44; 9; 5-2016; 4163-4173
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