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dc.contributor.author Danielli, Mauro
dc.contributor.author Marinelli, Raul Alberto
dc.date.available 2018-07-17T14:37:56Z
dc.date.issued 2016-02
dc.identifier.citation Danielli, Mauro; Marinelli, Raul Alberto; Lipid-based transfection reagents can interfere with cholesterol biosynthesis; Academic Press Inc Elsevier Science; Analytical Biochemistry; 495; 2-2016; 1-2
dc.identifier.issn 0003-2697
dc.identifier.uri http://hdl.handle.net/11336/52370
dc.description.abstract Lipid-based transfection reagents are widely used for delivery of small interfering RNA into cells. We examined whether the commonly used commercial transfection reagents DharmaFECT-4 and Lipofectamine 2000 can interfere with lipid metabolism by studying cholesterogenesis. Cholesterol de novo synthesis from [14C]acetate was assessed in human hepatocyte-derived Huh-7 cells. The results revealed that DharmaFECT, but not Lipofectamine, markedly inhibited cholesterol biosynthesis by approximately 70%. Cell viability was not significantly altered. These findings suggest that caution is required in the choice of certain lipid-based transfection reagents for gene silencing experiments, particularly when assessing cholesterol metabolism.
dc.format application/pdf
dc.language.iso eng
dc.publisher Academic Press Inc Elsevier Science
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject CHOLESTEROL
dc.subject HUH-7 CELLS
dc.subject TRANSFECTION REAGENTS
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Lipid-based transfection reagents can interfere with cholesterol biosynthesis
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-07-17T14:04:31Z
dc.journal.volume 495
dc.journal.pagination 1-2
dc.journal.pais Estados Unidos
dc.description.fil Fil: Danielli, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; Argentina
dc.description.fil Fil: Marinelli, Raul Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; Argentina
dc.journal.title Analytical Biochemistry
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ab.2015.11.008
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003269715005345
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)