Mostrar el registro sencillo del ítem
dc.contributor.author
Salido, Ezequiel Martin
dc.contributor.author
Dorfman, Damián
dc.contributor.author
Bordone, Melina Paula
dc.contributor.author
Chianelli, Monica Silvia
dc.contributor.author
González Fleitas, María Florencia
dc.contributor.author
Rosenstein, Ruth Estela
dc.date.available
2015-05-22T18:59:37Z
dc.date.issued
2013-04
dc.identifier.citation
Salido E; Dorfman D; Bordone, Melina; Chianelli Mónica S; González Fleitas Mf; Rosenstein Re; Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage; Public Library Science; Plos One; 8; 4; 2013-4; 61656-61668;
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/521
dc.description.abstract
Retinal ischemia could provoke blindness. At present, there is no effective treatment against retinal ischemic damage.
Strong evidence supports that glutamate is implicated in retinal ischemic damage. We investigated whether a brief period
of global or ocular hypothermia applied 24 h before ischemia (i.e. hypothermic preconditioning, HPC) protects the retina
from ischemia/reperfusion damage, and the involvement of glutamate in the retinal protection induced by HPC. For this
purpose, ischemia was induced by increasing intraocular pressure to 120 mm Hg for 40 min. One day before ischemia,
animals were submitted to global or ocular hypothermia (33uC and 32uC for 20 min, respectively) and fourteen days after
ischemia, animals were subjected to electroretinography and histological analysis. Global or ocular HPC afforded significant functional (electroretinographic) protection in eyes exposed to ischemia/reperfusion injury. A marked alteration of the retinal structure and a decrease in retinal ganglion cell number were observed in ischemic retinas, whereas global or ocular HPC significantly preserved retinal structure and ganglion cell count. Three days after ischemia, a significant decrease in retinal glutamate uptake and glutamine synthetase activity was observed, whereas ocular HPC prevented the effect of ischemia on these parameters. The intravitreal injection of supraphysiological levels of glutamate induced alterations in retinal function and histology which were significantly prevented by ocular HPC. These results support that global or ocular HPC significantly protected retinal function and histology from ischemia/reperfusion injury, probably through a glutamatedependent mechanism.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ischemia Retinal
dc.subject
Preconditioning
dc.subject
Hypothermia
dc.subject.classification
Ciencias Médicas y de la Salud
dc.subject.classification
Medicina Básica
dc.subject.classification
Neurociencias (incluye Psicofiosiología)
dc.title
Global and Ocular Hypothermic Preconditioning Protect the Rat Retina from Ischemic Damage
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
8
dc.journal.number
4
dc.journal.pagination
61656-61668
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Salido E.
dc.description.fil
Fil: Dorfman D.
dc.description.fil
Fil: Bordone, Melina.
dc.description.fil
Fil: Chianelli Mónica S.
dc.description.fil
Fil: González Fleitas Mf.
dc.description.fil
Fil: Rosenstein Re.
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/www.plosone.org
Archivos asociados