Artículo
Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms
Dasso, Maximiliano Carlos
; Pagotto, Romina María del Luján
; Pignataro, Omar Pedro
; Diez, Roberto Alejandro; Sales, María Elena
Fecha de publicación:
12/2011
Editorial:
Elsevier Science
Revista:
Biochimica et Biophysica Acta- General Subjects
ISSN:
0304-4165
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background: Amylase is synthesized in submandibular glands (SMG) and released into the oral cavity to degrade carbohydrates in the mouth. Bitter taste receptors (T2R) belong to the G-protein coupled receptor (GPCR) family and are expressed in the taste cells and also in the digestive tract. Methods: The activity of amylase secreted by murine SMG was measured, detecting maltose by Bernfeld's method. Amylase and T2R6 were detected by imunohistochemistry and Western blot. The expression of Ggustducin, Gi, and phospholipase Cβ2 was also studied by Western blot. cAMP levels were measured by radioimmunoassay and inositol monophosphate production was quantified by ELISA. Results: Theophylline, denatonium and cycloheximide exerted a dose-dependent inhibition on amylase secretion. This effect was reverted by preincubating SMG with an anti-Gαi antibody. cAMP production was increased by the same compounds, an effect that was also abrogated by an anti-Gαi antibody. Bitter compounds reduced inositol monophosphate formation in SMG and H-89, a protein kinase A inhibitor, reverted this action, revealing that this protein kinase down regulates phospholipase C activity. General significance: We demonstrated that theophylline, denatonium and cycloheximide inhibit salivary amylase secretion, activating an intracellular signaling pathway that involves cAMP and phospholipase C, that cross talks via protein kinase A. © 2011 Elsevier B.V.
Palabras clave:
Amylase
,
Bitter Agonist
,
Camp
,
Gi Protein
,
Inositol Monophosphate
,
Protein Kinase A
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Identificadores
Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Dasso, Maximiliano Carlos; Pagotto, Romina María del Luján; Pignataro, Omar Pedro; Diez, Roberto Alejandro; Sales, María Elena; Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms; Elsevier Science; Biochimica et Biophysica Acta- General Subjects; 1810; 12; 12-2011; 1212-1219
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