Artículo
In vitro plant regeneration of Alnus acuminata H.B.K. ssp. acuminata and its root nodulation by Frankia
Fecha de publicación:
03/2005
Editorial:
Springer
Revista:
Plant Cell, Tissue and Organ Culture
ISSN:
0167-6857
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A procedure for in vitro plant regeneration of Alnus acuminata from epicotyls with cotyledonary buds was developed using different media formulations with different growth regulators and carbon sources. The development of multiple buds on explants at the initiation step was obtained with MS at 1/2 strength with either 1 or 2 μM of BAP but not without it. Multiplication gave up to 15 elongating shoots by explant, the best medium being MS supplemented with vitamins from B5 medium, 1 μM of BAP and 87 mM sucrose. Rooting of about 88% occurred in the medium MS with 83 mM sucrose and 1 μM IBA. Alnus acuminata did not developed well on WPM. Roots of in vitro propagated plants were nodulated by Alnus-infective Frankia. The root nodules show a typical alder root nodule anatomy and differentiation pattern and effectively fixed nitrogen. Rhamnaceae-infective Frankia did not nodulate in vitro cultivated Alnus acuminata suggesting that symbiotic recognition was not altered by in vitro regeneration of the plant.
Palabras clave:
Alnus Acuminata
,
Actinorhiza
,
In Vitro Culture
,
Nitrogen Fixation
,
Symbiosis
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - NORDESTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Articulos(IBONE)
Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Citación
Enrico, Roxana Judith; Ramírez, Silvia Patricia; Mroginski, Luis Amado; Wall, Luis Gabriel; In vitro plant regeneration of Alnus acuminata H.B.K. ssp. acuminata and its root nodulation by Frankia; Springer; Plant Cell, Tissue and Organ Culture; 80; 3; 3-2005; 343-346
Compartir
Altmétricas