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dc.contributor.author
Pereyra, Andrea Soledad
dc.contributor.author
Mykhaylyk, Olga
dc.contributor.author
Falomir Lockhart, Eugenia
dc.contributor.author
Taylor, Jackson Richard
dc.contributor.author
Delbono, Osvaldo
dc.contributor.author
Goya, Rodolfo Gustavo
dc.contributor.author
Plank, Christian
dc.contributor.author
Hereñú, Claudia Beatriz
dc.date.available
2018-07-10T20:50:01Z
dc.date.issued
2016-04
dc.identifier.citation
Pereyra, Andrea Soledad; Mykhaylyk, Olga; Falomir Lockhart, Eugenia; Taylor, Jackson Richard; Delbono, Osvaldo; et al.; Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells; OMICS International; Journal of Nanomedicine & Nanotechnology; 7; 2; 4-2016; 1-11
dc.identifier.issn
2157-7439
dc.identifier.uri
http://hdl.handle.net/11336/51671
dc.description.abstract
The goal of magnetic field-assisted gene transfer is to enhance internalization of exogenous nucleic acids by association with magnetic nanoparticles (MNPs). This technique named magnetofection is particularly useful in difficult-to-transfect cells. It is well known that human, mouse, and rat skeletal muscle cells suffer a maturation-dependent loss of susceptibility to Recombinant Adenoviral vector (RAd) uptake. In postnatal, fully differentiated myofibers, the expression of the primary Coxsackie and Adenoviral membrane receptor (CAR) is severely downregulated representing a main hurdle for the use of these vectors in gene transfer/therapy. Here we demonstrate that assembling of Recombinant Adenoviral vectors with suitable iron oxide MNPs into magneto-adenovectors (RAd-MNP) and further exposure to a gradient magnetic field enables to efficiently overcome transduction resistance in skeletal muscle cells. Expression of Green Fluorescent Protein and Insulin-like Growth Factor 1 was significantly enhanced after magnetofection with RAd-MNPs complexes in C2C12 myotubes in vitro and mouse skeletal muscle in vivo when compared to transduction with naked virus. These results provide evidence that magnetofection, mainly due to its membrane-receptor independent mechanism, constitutes a simple and effective alternative to current methods for gene transfer into traditionally hard-to-transfect biological models.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
OMICS International
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Gene Delivery
dc.subject
Skeletal Muscle
dc.subject
Magnetic Nanoparticles
dc.subject
Adenoviral Vectors
dc.subject.classification
Nano-materiales
dc.subject.classification
Nanotecnología
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-15T18:34:31Z
dc.journal.volume
7
dc.journal.number
2
dc.journal.pagination
1-11
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Pereyra, Andrea Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil
Fil: Mykhaylyk, Olga. Technische Universitat Munchen; Alemania
dc.description.fil
Fil: Falomir Lockhart, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil
Fil: Taylor, Jackson Richard. University Wake Forest; Estados Unidos
dc.description.fil
Fil: Delbono, Osvaldo. University Wake Forest; Estados Unidos
dc.description.fil
Fil: Goya, Rodolfo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil
Fil: Plank, Christian. Technische Universitat Munchen; Alemania
dc.description.fil
Fil: Hereñú, Claudia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.journal.title
Journal of Nanomedicine & Nanotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.4172/2157-7439.1000364
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/magnetofection-enhances-adenoviral-vectorbased-gene-delivery-inskeletal-muscle-cells-2157-7439-1000364.php?aid=71417


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