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dc.contributor.author
Jaureguiberry, María Soledad
dc.contributor.author
Tricerri, Maria Alejandra
dc.contributor.author
Sanchez, Susana A.
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Finarelli, Gabriela Sandra
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Montanaro, Mauro Aldo
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Prieto, Eduardo Daniel
dc.contributor.author
Rimoldi, Omar Jorge
dc.date.available
2016-04-12T18:26:37Z
dc.date.issued
2014-01
dc.identifier.citation
Jaureguiberry, María Soledad; Tricerri, Maria Alejandra; Sanchez, Susana A.; Finarelli, Gabriela Sandra; Montanaro, Mauro Aldo; et al.; Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases; Oxford University Press; Acta Biochimica Et Biophysica Sinica; 46; 4; 1-2014; 273-282
dc.identifier.issn
1005-9628
dc.identifier.uri
http://hdl.handle.net/11336/5161
dc.description.abstract
Experimental evidence has suggested that plasma membrane (PM)-associated signaling and hence cell metabolism and viability depend on lipid composition and organization. The aim of the present work is to develop a cell model to study the endogenous polyunsaturated fatty acids (PUFAs) effect on PM properties and analyze its influence on cholesterol (Chol) homeostasis. We have previously shown that by using a cell line over-expressing stearoyl-CoA-desaturase, membrane composition and organization coordinate cellular pathways involved in Chol efflux and cell viability by different mechanisms. Now, we expanded our studies to a cell model over-expressing both Δ5 and Δ6 desaturases, which resulted in a permanently higher PUFA content in PM. Furthermore, this cell line showed increased PM fluidity, Chol storage, and mitochondrial activity. In addition, human apolipoprotein A-I-mediated Chol removal was less efficient in these cells than in the corresponding control. Taken together, our results suggested that the cell functionality is preserved by regulating PM organization and Chol exportation and homeostasis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Membrane Heterogeneity
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Fatty Acid Desaturases
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Cholesterol Transport
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Cytotoxicity
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Human Apolipoprotein A-I
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-05-06 15:52:43.262787-03
dc.journal.volume
46
dc.journal.number
4
dc.journal.pagination
273-282
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Jaureguiberry, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina. Universidad Nacional de La Plata; Argentina
dc.description.fil
Fil: Tricerri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina. Universidad Nacional de La Plata; Argentina
dc.description.fil
Fil: Sanchez, Susana A.. University Of California At Irvine. Laboratory of Fluorescence Dynamics; Estados Unidos
dc.description.fil
Fil: Finarelli, Gabriela Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina. Universidad Nacional de La Plata; Argentina
dc.description.fil
Fil: Montanaro, Mauro Aldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina. Universidad Nacional de La Plata; Argentina
dc.description.fil
Fil: Prieto, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina
dc.description.fil
Fil: Rimoldi, Omar Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina. Universidad Nacional de La Plata; Argentina
dc.journal.title
Acta Biochimica Et Biophysica Sinica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/PMC3967655
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://abbs.oxfordjournals.org/content/46/4/273.long
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/abbs/gmt155
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967655/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1093/abbs/gmt155
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