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dc.contributor.author
Gangoiti, Maria Virginia
dc.contributor.author
Anbinder, Pablo Sebastián
dc.contributor.author
Cortizo, Ana Maria
dc.contributor.author
Mccarthy, Antonio Desmond
dc.date.available
2016-04-12T13:59:12Z
dc.date.issued
2013-09
dc.identifier.citation
Gangoiti, Maria Virginia; Anbinder, Pablo Sebastián; Cortizo, Ana Maria; Mccarthy, Antonio Desmond; Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate; Elsevier; Acta Histochemica; 115; 7; 9-2013; 649-657
dc.identifier.issn
0065-1281
dc.identifier.uri
http://hdl.handle.net/11336/5142
dc.description.abstract
Advanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients with Diabetes mellitus, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated the in vitro alterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5 M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8 M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Osteoblast
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Advanced Glycation Endproducts
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Bisphosphonate
dc.subject
Apoptosis
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Actin Cytoskeleton
dc.subject
Environmental Scanning Electron Microscopy
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-05-06 15:52:43.262787-03
dc.journal.volume
115
dc.journal.number
7
dc.journal.pagination
649-657
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Gangoiti, Maria Virginia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; Argentina
dc.description.fil
Fil: Anbinder, Pablo Sebastián. Universidad Nacional de la Plata. Facultad de Ingenieria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
dc.description.fil
Fil: Cortizo, Ana Maria. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; Argentina
dc.description.fil
Fil: Mccarthy, Antonio Desmond. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; Argentina
dc.journal.title
Acta Histochemica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0065128113000184
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.acthis.2013.01.004
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.acthis.2013.01.004


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