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dc.contributor.author
Grillo, Claudia Alejandra
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dc.contributor.author
Alvarez, Florencia
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dc.contributor.author
Fernandez Lorenzo, Monica Alicia
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dc.date.available
2016-04-11T20:49:44Z
dc.date.issued
2014-03-02
dc.identifier.citation
Grillo, Claudia Alejandra; Alvarez, Florencia; Fernandez Lorenzo, Monica Alicia; Cellular response to rare earth mixtures (La and Gd) as components of degradable Mg alloys for medical applications; Elsevier; Colloids And Surfaces B: Biointerfaces; 117; 02-3-2014; 312-321
dc.identifier.issn
0927-7765
dc.identifier.uri
http://hdl.handle.net/11336/5134
dc.description.abstract
Rare earth (RE) elements have been proposed to improve the corrosion resistance of degradable Mg alloys for medical applications. However, good biocompatibility of the elements released by Mg alloys during degradation is essential for their use in implants. Most studies are focused on material science and engineering aspects, but the effects of ions released at the biological interface are not frequently addressed. The aim of this study was to contribute to the knowledge of in vitro toxicological effects of two RE Mg-alloying elements, La and Gd, as individual ions and in mixtures with and without Mg ions. Different combinations (Mg + Gd, Mg + La, and Mg + Gd + La) were used to evaluate their possible synergistic effects on CHO-K1 cells. Two sets of experiments were designed to assess (1) the cyto-genotoxic effect of La and Gd ions by neutral red (NR) technique, Reduction of tetrazolium salt (MTT), Viability with Acridine Orange staining, Clonogenic test, and Comet assay; and, (2) the possible synergistic toxicological effect of La and Gd ions in mixtures, and the influence of osmolarity increase on cellular response. Cytotoxic effects of RE were found at concentrations ≥200 μM RE while DNA damage was detected for doses ≥1500 μM and ≥1600 μM for La and Gd, respectively. When mixtures of ions were evaluated, neither synergistic cytotoxic effects nor biological damage related to osmolarity increase were detected.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Cytotoxicity
dc.subject
Rare Earth
dc.subject
Magnesium
dc.subject
Dna Damage
dc.subject
Lanthanum
dc.subject.classification
Biomateriales
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dc.subject.classification
Biotecnología de la Salud
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Cellular response to rare earth mixtures (La and Gd) as components of degradable Mg alloys for medical applications
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-05-06 15:52:43.262787-03
dc.journal.volume
117
dc.journal.pagination
312-321
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Grillo, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
dc.description.fil
Fil: Alvarez, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
dc.description.fil
Fil: Fernandez Lorenzo, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ingenieria; Argentina
dc.journal.title
Colloids And Surfaces B: Biointerfaces
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/24667077
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776514000976
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2014.02.030
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.colsurfb.2014.02.030
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