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dc.contributor.author
Matamoros, Manuel A.
dc.contributor.author
Saiz, Ana
dc.contributor.author
Peñuelas, Maria
dc.contributor.author
Bustos Sanmamed, Maria del Pilar
dc.contributor.author
Mulet, Jose M.
dc.contributor.author
Barja, Maria V.
dc.contributor.author
Rouhier, Nicolas
dc.contributor.author
Moore, Marten
dc.contributor.author
James, Euan K.
dc.contributor.author
Dietz, Karl Josef
dc.contributor.author
Becana, Manuel
dc.date.available
2018-07-03T16:54:54Z
dc.date.issued
2015-05
dc.identifier.citation
Matamoros, Manuel A.; Saiz, Ana; Peñuelas, Maria; Bustos Sanmamed, Maria del Pilar; Mulet, Jose M.; et al.; Function of glutathione peroxidases in legume root nodules; Oxford University Press; Journal of Experimental Botany; 66; 10; 5-2015; 2979-2990
dc.identifier.issn
0022-0957
dc.identifier.uri
http://hdl.handle.net/11336/51047
dc.description.abstract
Glutathione peroxidases (Gpxs) are antioxidant enzymes not studied so far in legume nodules, despite the fact that reactive oxygen species are produced at different steps of the symbiosis. The function of two Gpxs that are highly expressed in nodules of the model legume Lotus japonicus was examined. Gene expression analysis, enzymatic and nitrosylation assays, yeast cell complementation, in situ mRNA hybridization, immunoelectron microscopy, and LjGpx-green fluorescent protein (GFP) fusions were used to characterize the enzymes and to localize each transcript and isoform in nodules. The LjGpx1 and LjGpx3 genes encode thioredoxin-dependent phospholipid hydroperoxidases and are differentially regulated in response to nitric oxide (NO) and hormones. LjGpx1 and LjGpx3 are nitrosylated in vitro or in plants treated with S-nitrosoglutathione (GSNO). Consistent with the modification of the peroxidatic cysteine of LjGpx3, in vitro assays demonstrated that this modification results in enzyme inhibition. The enzymes are highly expressed in the infected zone, but the LjGpx3 mRNA is also detected in the cortex and vascular bundles. LjGpx1 is localized to the plastids and nuclei, and LjGpx3 to the cytosol and endoplasmic reticulum. Based on yeast complementation experiments, both enzymes protect against oxidative stress, salt stress, and membrane damage. It is concluded that both LjGpxs perform major antioxidative functions in nodules, preventing lipid peroxidation and other oxidative processes at different subcellular sites of vascular and infected cells. The enzymes are probably involved in hormone and NO signalling, and may be regulated through nitrosylation of the peroxidatic cysteine essential for catalytic function.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Antioxidants
dc.subject
Glutathione Peroxidases
dc.subject
Legume Nodules
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Lotus Japonicus
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Nitric Oxide
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Reactive Oxygen Species
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S-Nitrosylation
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Function of glutathione peroxidases in legume root nodules
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-07-03T14:22:35Z
dc.journal.volume
66
dc.journal.number
10
dc.journal.pagination
2979-2990
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Matamoros, Manuel A.. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Saiz, Ana. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Peñuelas, Maria. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Bustos Sanmamed, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institut des Sciences du Végétal; Francia
dc.description.fil
Fil: Mulet, Jose M.. Consejo Superior de Investigaciones Científicas; España. Universidad Politécnica de Valencia; España
dc.description.fil
Fil: Barja, Maria V.. Consejo Superior de Investigaciones Científicas; España. Universidad Politécnica de Valencia; España
dc.description.fil
Fil: Rouhier, Nicolas. Universite de Lorraine; Francia. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Moore, Marten. Bielefeld University; Alemania
dc.description.fil
Fil: James, Euan K.. The James Hutton Institute; Reino Unido
dc.description.fil
Fil: Dietz, Karl Josef. Bielefeld University; Alemania
dc.description.fil
Fil: Becana, Manuel. Consejo Superior de Investigaciones Científicas; España
dc.journal.title
Journal of Experimental Botany
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1093/jxb/erv066
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/66/10/2979/533365
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