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Artículo

Selected SNARE proteins are essential for the polarized membrane insertion of igf-1 receptor and the regulation of initial axonal outgrowth in neurons

Grassi, Diego JavierIcon ; Plonka, Florentyna Bustos; Oksdath Mansilla, MarianaIcon ; Nieto Guil, Alvaro FernandoIcon ; Sosa, Lucas JavierIcon ; Quiroga, SantiagoIcon
Fecha de publicación: 09/2015
Editorial: Nature Publishing Group
Revista: Cell Discovery
ISSN: 2056-5968
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

The establishment of polarity necessitates initial axonal outgrowth and, therefore, the addition of new membrane to the axon's plasmalemma. Axolemmal expansion occurs by exocytosis of plasmalemmal precursor vesicles (PPVs) primarily at the neuronal growth cone. Little is known about the SNAREs family proteins involved in the regulation of PPV fusion with the neuronal plasmalemma at early stages of differentiation. We show here that five SNARE proteins (VAMP2, VAMP4, VAMP7, Syntaxin6 and SNAP23) were expressed by hippocampal pyramidal neurons before polarization. Expression silencing of three of these proteins (VAMP4, Syntaxin6 and SNAP23) repressed axonal outgrowth and the establishment of neuronal polarity, by inhibiting IGF-1 receptor exocytotic polarized insertion, necessary for neuronal polarization. In addition, stimulation with IGF-1 triggered the association of VAMP4, Syntaxin6 and SNAP23 to vesicular structures carrying the IGF-1 receptor and overexpression of a negative dominant form of Syntaxin6 significantly inhibited exocytosis of IGF-1 receptor containing vesicles at the neuronal growth cone. Taken together, our results indicated that VAMP4, Syntaxin6 and SNAP23 functions are essential for regulation of PPV exocytosis and the polarized insertion of IGF-1 receptor and, therefore, required for initial axonal elongation and the establishment of neuronal polarity.
Palabras clave: Igf-1 Receptor , Membrane Expansion , Neuronal Differentiation , Neuronal Polarization , Sintaxyn6 , Snap23 , Vamp4
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/50799
DOI: http://dx.doi.org/10.1038/celldisc.2015.23
URL: https://www.nature.com/articles/celldisc201523
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Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Citación
Grassi, Diego Javier; Plonka, Florentyna Bustos; Oksdath Mansilla, Mariana; Nieto Guil, Alvaro Fernando; Sosa, Lucas Javier; et al.; Selected SNARE proteins are essential for the polarized membrane insertion of igf-1 receptor and the regulation of initial axonal outgrowth in neurons; Nature Publishing Group; Cell Discovery; 1; 9-2015; 1-15
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