Show simple item record

dc.contributor.author Cambiagno, Damián Alejandro
dc.contributor.author Lonez, Caroline
dc.contributor.author Ruysschaert, Jean Marie
dc.contributor.author Alvarez, Maria Elena
dc.date.available 2018-06-29T18:21:59Z
dc.date.issued 2015-12
dc.identifier.citation Cambiagno, Damián Alejandro; Lonez, Caroline; Ruysschaert, Jean Marie; Alvarez, Maria Elena; The synthetic cationic lipid diC14 activates a sector of the Arabidopsis defence network requiring endogenous signalling components; Wiley Blackwell Publishing, Inc; Molecular Plant Pathology; 16; 9; 12-2015; 963-972
dc.identifier.issn 1464-6722
dc.identifier.uri http://hdl.handle.net/11336/50741
dc.description.abstract Natural and synthetic elicitors have contributed significantly to the study of plant immunity. Pathogen-derived proteins and carbohydrates that bind to immune receptors, allow the fine dissection of certain defence pathways. Lipids of a different nature that act as defence elicitors, have also been studied, but their specific effects have been less well characterized, and their receptors have not been identified. In animal cells, nanoliposomes of the synthetic cationic lipid 3-tetradecylamino-tert-butyl-N-tetradecylpropionamidine (diC14) activate the TLR4-dependent immune cascade. Here, we have investigated whether this lipid induces Arabidopsis defence responses. At the local level, diC14 activated early and late defence gene markers (FRK1, WRKY29, ICS1 and PR1), acting in a dose-dependent manner. This lipid induced the salicylic acid (SA)-dependent, but not jasmonic acid (JA)-dependent, pathway and protected plants against Pseudomonas syringae pv. tomato (Pst), but not Botrytis cinerea. diC14 was not toxic to plant or pathogen, and potentiated pathogen-induced callose deposition. At the systemic level, diC14 induced PR1 expression and conferred resistance against Pst. diC14-induced defence responses required the signalling protein EDS1, but not NDR1. Curiously, the lipid-induced defence gene expression was lower in the fls2/efr/cerk1 triple mutant, but still unchanged in the single mutants. The amidine headgroup and chain length were important for its activity. Given the robustness of the responses triggered by diC14, its specific action on a defence pathway and the requirement for well-known defence components, this synthetic lipid is emerging as a useful tool to investigate the initial events involved in plant innate immunity.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley Blackwell Publishing, Inc
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ARABIDOPSIS
dc.subject LIPID ELICITORS
dc.subject LOCAL AND SYSTEMIC DEFENCES
dc.subject PAMP/DAMP
dc.subject SA- AND JA-DEPENDENT PATHWAYS
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title The synthetic cationic lipid diC14 activates a sector of the Arabidopsis defence network requiring endogenous signalling components
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-06-29T16:44:04Z
dc.identifier.eissn 1364-3703
dc.journal.volume 16
dc.journal.number 9
dc.journal.pagination 963-972
dc.journal.pais Reino Unido
dc.journal.ciudad Londres
dc.description.fil Fil: Cambiagno, Damián Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.description.fil Fil: Lonez, Caroline. Université Libre de Bruxelles; Bélgica
dc.description.fil Fil: Ruysschaert, Jean Marie. Université Libre de Bruxelles; Bélgica
dc.description.fil Fil: Alvarez, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.journal.title Molecular Plant Pathology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/mpp.12252/abstract
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/mpp.12252
dc.conicet.fuente unificacion


Archivos asociados

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)