Artículo
Copper redox chemistry of plant frataxins
Sánchez, Manu; Palacios, Oscar; Buchensky, Celeste
; Sabio, Laura; Gomez Casati, Diego Fabian
; Pagani, María Ayelén
; Capdevila, Mercè; Atrian, Silvia; Dominguez Vera, Jose M.
Fecha de publicación:
03/2018
Editorial:
Elsevier Science Inc
Revista:
Journal of Inorganic Biochemistry
ISSN:
0162-0134
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The presence of a conserved cysteine residue in the C-terminal amino acid sequences of plant frataxins differentiates these frataxins from those of other kingdoms and may be key in frataxin assembly and function. We report a full study on the ability of Arabidopsis (AtFH) and Zea mays (ZmFH-1 and ZmFH-2) frataxins to assemble into disulfide-bridged dimers by copper-driven oxidation and to revert to monomers by chemical reduction. We monitored the redox assembly-disassembly process by electrospray ionization mass spectrometry, electrophoresis, UV–Vis spectroscopy, and fluorescence measurements. We conclude that plant frataxins AtFH, ZmFH-1 and ZmFH-2 are oxidized by Cu2 + and exhibit redox cysteine monomer – cystine dimer interexchange. Interestingly, the tendency to interconvert is not the same for each protein. Through yeast phenotypic rescue experiments, we show that plant frataxins are important for plant survival under conditions of excess copper, indicating that these proteins might be involved in copper metabolism.
Palabras clave:
Copper Chemistry of Frataxin
,
Plant Frataxins
,
Protein Assembly
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Articulos(CEFOBI)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Citación
Sánchez, Manu; Palacios, Oscar; Buchensky, Celeste; Sabio, Laura; Gomez Casati, Diego Fabian; et al.; Copper redox chemistry of plant frataxins; Elsevier Science Inc; Journal of Inorganic Biochemistry; 180; 3-2018; 135-140
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