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dc.contributor.author
Cho, Won Jin
dc.contributor.author
Oliveira, Daniel S.M.
dc.contributor.author
Najy, Abdo J.
dc.contributor.author
Mainetti, Leandro Ernesto
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dc.contributor.author
Aoun, Hussein D.
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Cher, Michael L.
dc.contributor.author
Heath, Elisabeth
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Kim, Hyeong-Reh C.
dc.contributor.author
Bonfil, Ricardo Daniel
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dc.date.available
2018-06-28T13:56:52Z
dc.date.issued
2016-03
dc.identifier.citation
Cho, Won Jin; Oliveira, Daniel S.M.; Najy, Abdo J.; Mainetti, Leandro Ernesto; Aoun, Hussein D.; et al.; Gene expression analysis of bone metastasis and circulating tumor cells from metastatic castrate-resistant prostate cancer patients; BioMed Central; Journal Of Translational Medicine; 14; 1; 3-2016; 1-12
dc.identifier.issn
1479-5876
dc.identifier.uri
http://hdl.handle.net/11336/50321
dc.description.abstract
Background: Characterization of genes linked to bone metastasis is critical for identification of novel prognostic or predictive biomarkers and potential therapeutic targets in metastatic castrate-resistant prostate cancer (mCRPC). Although bone marrow core biopsies (BMBx) can be obtained for gene profiling, the procedure itself is invasive and uncommon practice in mCRPC patients. Conversely, circulating tumor cells (CTCs), which are likely to stem from bone metastases, can be isolated from blood. The goals of this exploratory study were to establish a sensitive methodology to analyze gene expression in BMBx and CTCs, and to determine whether the presence or absence of detectable gene expression is concordant in matching samples from mCRPC patients. Methods: The CellSearch® platform was used to enrich and enumerate CTCs. Low numbers of PC3 prostate cancer (PCa) cells were spiked into normal blood to assess cell recovery rate. RNA extracted from recovered PC3 cells was amplified using an Eberwine-based procedure to obtain antisense mRNA (aRNA), and assess the linearity of the RNA amplification method. In this pilot study, RNAs extracted from CTCs and PCa cells microdissected from formalin-fixed paraffin-embedded BMBx, were amplified to obtain aRNA and assess the expression of eight genes functionally relevant to PCa bone metastasis using RT-PCR. Results: RNAs were successfully extracted from as few as 1-5 PCa cells in blood samples. The relative expression levels of reference genes were maintained after RNA amplification. The integrity of the amplified RNA was also demonstrated by RT-PCR analysis using primer sets that target the 5'-end, middle, and 3'-end of reference mRNA. We found that in 21 out of 28 comparisons, the presence or absence of detectable gene expression in CTCs and PCa cells microdissected from single bone lesions of the same patients was concordant. Conclusions: This exploratory analysis suggests that aRNA amplification through in vitro transcription may be useful as a method to detect gene expression in small numbers of CTCs and tumor cells microdissected from bone metastatic lesions. In some cases, gene expression in CTCs and BMBxs was not concordant, raising questions about using CTC gene expression to make clinical decisions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bone Metastasis
dc.subject
Circulating Tumor Cells
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Gene Expression
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Laser Capture Microdissection
dc.subject
Prostate Cancer
dc.subject.classification
Inmunología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Gene expression analysis of bone metastasis and circulating tumor cells from metastatic castrate-resistant prostate cancer patients
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-26T22:33:59Z
dc.journal.volume
14
dc.journal.number
1
dc.journal.pagination
1-12
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Cho, Won Jin. Wayne State University; Estados Unidos
dc.description.fil
Fil: Oliveira, Daniel S.M.. Wayne State University; Estados Unidos
dc.description.fil
Fil: Najy, Abdo J.. Wayne State University; Estados Unidos
dc.description.fil
Fil: Mainetti, Leandro Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Wayne State University; Estados Unidos
dc.description.fil
Fil: Aoun, Hussein D.. Wayne State University; Estados Unidos
dc.description.fil
Fil: Cher, Michael L.. Wayne State University; Estados Unidos
dc.description.fil
Fil: Heath, Elisabeth. Wayne State University; Estados Unidos
dc.description.fil
Fil: Kim, Hyeong-Reh C.. Wayne State University; Estados Unidos
dc.description.fil
Fil: Bonfil, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Wayne State University; Estados Unidos
dc.journal.title
Journal Of Translational Medicine
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1186/s12967-016-0829-5
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-016-0829-5
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