Show simple item record

dc.contributor.author Villegas Gabutti, Carlos Mauricio
dc.contributor.author Pennacchio, Gisela Erika
dc.contributor.author Vivas, Laura Marta
dc.contributor.author Jahn, Graciela Alma
dc.contributor.author Soaje, Marta
dc.date.available 2018-06-26T22:40:41Z
dc.date.issued 2018-05
dc.identifier.citation Villegas Gabutti, Carlos Mauricio; Pennacchio, Gisela Erika; Vivas, Laura Marta; Jahn, Graciela Alma; Soaje, Marta; Role of Oxytocin in Prolactin Secretion during Late Pregnancy; Karger; Neuroendocrinology; 106; 4; 5-2018; 324-334
dc.identifier.issn 0028-3835
dc.identifier.uri http://hdl.handle.net/11336/50207
dc.description.abstract Background/Aims: During late pregnancy, the blockade of progesterone action by mifepristone (Mp) treatment induces a dopaminergic tone fall that enables naloxone (NAL) administration to release pituitary prolactin (PRL). We determined whether oxytocin (OT), which stimulates PRL secretion acting directly on anterior pituitary lactotrophs, mediates the stimulatory action of Mp and NAL on PRL secretion during late pregnancy. Methods: On day 19 of pregnancy, circulating and pituitary OT and PRL levels were measured by radioimmunoassay, 10, 20, and 30 min after NAL (given at 17:30 h) in rats pretreated with Mp (at 08:00 h). Pituitary OT receptor (OTR) expression in Mp-treated rats was evaluated by RT-PCR. Activation of OT neurons in Mp-NAL-treated rats was measured counting double immunoreactive neurons for Fos and OT (Fos-OT-ir) in supraoptic nuclei (SON), and medial (PaMM) and lateral magnocellular divisions of paraventricular nuclei. Results: Elevated serum OT and decreased pituitary OT were observed 10 min after NAL administration in both vehicle-and Mp-treated rats. This PRL increase was prevented by previous i.p. administration of an OTR antagonist, but intracerebroventricular OT administration was ineffective. Mp increased pituitary OTR expression at 18:00 h. Only Mp-NAL increased Fos-OT-ir neurons in the PaMM and SON. Conclusions: These findings suggest that PRL secretion induced by Mp-NAL treatment is preceded by OT release. These results, together with the activation of hypothalamic OT neurons and the higher expression of pituitary OTR, support the hypothesis that, during late pregnancy, OT may act at the pituitary level to facilitate PRL secretion if the inhibitory action of progesterone is blocked.
dc.format application/pdf
dc.language.iso eng
dc.publisher Karger
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject MIFEPRISTONE
dc.subject NALOXONE
dc.subject OXYTOCIN
dc.subject PREGNANCY
dc.subject PROLACTIN
dc.subject RAT
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Role of Oxytocin in Prolactin Secretion during Late Pregnancy
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-06-18T19:11:35Z
dc.journal.volume 106
dc.journal.number 4
dc.journal.pagination 324-334
dc.journal.pais Suiza
dc.journal.ciudad Basel
dc.description.fil Fil: Villegas Gabutti, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil Fil: Pennacchio, Gisela Erika. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil Fil: Vivas, Laura Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.description.fil Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil Fil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.journal.title Neuroendocrinology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/?doi=10.1159/000480669
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1159/000480669
dc.conicet.fuente Crossref


Archivos asociados

Icon
Blocked Acceso no disponible

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)