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Artículo

Reactive oxygen species generated in chloroplasts contribute to tobacco leaf infection by the necrotrophic fungus Botrytis cinerea

Rossi, Franco RubénIcon ; Krapp, Adriana del RosarioIcon ; Bisaro, Fabiana; Maiale, Santiago JavierIcon ; Pieckenstain, Fernando LuisIcon ; Carrillo, Nestor JoseIcon
Fecha de publicación: 12/2017
Editorial: Wiley Blackwell Publishing, Inc
Revista: Plant Journal
ISSN: 0960-7412
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Agricultura

Resumen

Reactive oxygen species (ROS) play fundamental roles in plant responses to pathogen infection, including modulation of cell death processes and defense-related gene expression. Cell death triggered as part of the hypersensitive response enhances resistance to biotrophic pathogens, but favors the virulence of necrotrophs. Even though the involvement of ROS in the orchestration of defense responses is well established, the relative contribution of specific subcellular ROS sources to plant resistance against microorganisms with different pathogenesis strategies is not completely known. The aim of this work was to investigate the role of chloroplastic ROS in plant defense against a typical necrotrophic fungus, Botrytis cinerea. For this purpose, we used transgenic Nicotiana tabacum (tobacco) lines expressing a plastid-targeted cyanobacterial flavodoxin (pfld lines), which accumulate lower chloroplastic ROS in response to different stresses. Tissue damage and fungal growth were significantly reduced in infected leaves of pfld plants, as compared with infected wild-type (WT) counterparts. ROS build-up triggered by Botrytis infection and associated with chloroplasts was significantly decreased (70–80%) in pfld leaves relative to the wild type. Phytoalexin accumulation and expression of pathogenesis-related genes were induced to a lower degree in pfld plants than in WT siblings. The impact of fungal infection on photosynthetic activity was also lower in pfld leaves. The results indicate that chloroplast-generated ROS play a major role in lesion development during Botrytis infection. This work demonstrates that the modulation of chloroplastic ROS levels by the expression of a heterologous antioxidant protein can provide a significant degree of protection against a canonical necrotrophic fungus.
Palabras clave: Botrytis Cinerea , Chloroplastic Ros , Flavodoxin , Necrotrophs , Nicotiana Tabacum , Plant–Microbe Interactions
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/50170
DOI: http://dx.doi.org/10.1111/tpj.13718
URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.13718
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Citación
Rossi, Franco Rubén; Krapp, Adriana del Rosario; Bisaro, Fabiana; Maiale, Santiago Javier; Pieckenstain, Fernando Luis; et al.; Reactive oxygen species generated in chloroplasts contribute to tobacco leaf infection by the necrotrophic fungus Botrytis cinerea; Wiley Blackwell Publishing, Inc; Plant Journal; 92; 5; 12-2017; 761-773
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