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Artículo

Chromatographic matrix based on hydrogel-coated reticulated polyurethane foams, prepared by gamma irradiation

Sanchez, Mirna LorenaIcon ; Giménez, Claudia Y.; Delgado, Juan FranciscoIcon ; Martínez, Leandro JuliánIcon ; Grasselli, MarianoIcon
Fecha de publicación: 12/2017
Editorial: Pergamon-Elsevier Science Ltd
Revista: Radiation Physics and Chemistry (Oxford)
ISSN: 0969-806X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Recubrimientos y Películas

Resumen

Novel chromatographic materials for protein purification with high adsorption capacity and fouling resistance are highly demanded to improve downstream processes. Here, we describe a novel adsorptive material based on reticulated polyurethane foam (rPUF) coated with a functional hydrogel layer. rPUF provides physical rigidity through its macroscopic structure, whereas the hydrogel layer provides capacity to adsorb proteins by specific interactions. The hydrogel coating process was performed by the dip-coating method, using a polyvinyl alcohol (PVA) solution. The PVA hydrogel was linked to the rPUF material by using a radiation-induced crosslinking process in aqueous ethanol solution. The ethanol in the solvent mixture allowed a balance between PVA swelling and PVA dissolution during the irradiation step. The resulting material showed higher thermal stability than the non-irradiated one. In addition, a simultaneous radiation-induced grafting polymerization (SRIGP) was done by simple addition of glycidyl methacrylate monomer into the irradiation solution. In a further step, sulfonic ligands were included specifically in the hydrogel layer, which contained around 200% of PVA respect to the original rPUF. Materials were characterized by FT-IR, thermogravimetric analysis, SEM microscopy and EDX analysis. The cation-exchange rPUF material was functionally characterized by the Langmuir isotherm and a dynamic adsorption experiment to analyze the chromatographic properties for protein purification processes.
Palabras clave: Chromatography , Protein Adsorption , Pva Hydrogel , Radiation-Induced Polymerization
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/50159
URL: https://www.sciencedirect.com/science/article/pii/S0969806X17300191
DOI: http://dx.doi.org/10.1016/j.radphyschem.2017.08.006
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(IMBICE)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA CELULAR (I)
Citación
Sanchez, Mirna Lorena; Giménez, Claudia Y.; Delgado, Juan Francisco; Martínez, Leandro Julián; Grasselli, Mariano; Chromatographic matrix based on hydrogel-coated reticulated polyurethane foams, prepared by gamma irradiation; Pergamon-Elsevier Science Ltd; Radiation Physics and Chemistry (Oxford); 141; 12-2017; 300-311
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