Mostrar el registro sencillo del ítem
dc.contributor.author
García, Paula
dc.contributor.author
Furlan, Ricardo Luis Eugenio
dc.date.available
2018-06-25T18:23:41Z
dc.date.issued
2015-07
dc.identifier.citation
García, Paula; Furlan, Ricardo Luis Eugenio; Multiresponse Optimisation Applied to the Development of a TLC Autography for the Detection of Tyrosinase Inhibitors; John Wiley & Sons Ltd; Phytochemical Analysis; 26; 4; 7-2015; 287-292
dc.identifier.issn
0958-0344
dc.identifier.uri
http://hdl.handle.net/11336/49967
dc.description.abstract
Introduction: Autographic methods are useful tools to detect bioactive compounds in complex matrixes. Experimental design and optimisation techniques were implemented for the development of an autographic assay suitable for the detection of tyrosinase inhibitors. Objectives: To develop an autographic assay to detect tyrosinase inhibitors using gel entrapped enzyme, experimental design and response surface methodology (RSM) to optimise conditions with a minimum number of experiments. Methods: Gel entrapment was used for the assay and the effects of four factors on the sensitivity and the detection limit for known inhibitors of the enzyme were evaluated. The factors were: tyrosinase amount (TA), L‐tyrosine amount (LTA), incubation time and incubation temperature. Results: The assay allowed the detection of kojic acid in an extract of Calamagrostis viridiflavescens (Poir.) Steud spiked with 0.1% w/w. Conclusion: The developed assay is able to detect tyrosinase inhibitors present in complex matrixes in a reproducible way.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Dermatological Disorders
dc.subject
Herbal Drugs
dc.subject
Response Surface Methodology
dc.subject
Tlc-Bioautography
dc.subject
Tyrosinase Inhibition
dc.subject.classification
Otras Ciencias Químicas
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Multiresponse Optimisation Applied to the Development of a TLC Autography for the Detection of Tyrosinase Inhibitors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-25T12:39:36Z
dc.journal.volume
26
dc.journal.number
4
dc.journal.pagination
287-292
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: García, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Furlan, Ricardo Luis Eugenio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Phytochemical Analysis
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/pca.2562
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.2562
Archivos asociados