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dc.contributor.author
Alvarez Olmedo, Daiana Gisela
dc.contributor.author
Biaggio, Veronica Silvina
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Koumbadinga, Geremy A.
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Gomez, Nidia Noemí
dc.contributor.author
Shi, Chunhua
dc.contributor.author
Ciocca, Daniel Ramon
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Batulan, Zarah
dc.contributor.author
Fanelli, Mariel Andrea
dc.contributor.author
O´Brien, Edward R.
dc.date.available
2018-06-22T20:40:11Z
dc.date.issued
2017-05
dc.identifier.citation
Alvarez Olmedo, Daiana Gisela; Biaggio, Veronica Silvina; Koumbadinga, Geremy A.; Gomez, Nidia Noemí; Shi, Chunhua; et al.; Recombinant heat shock protein 27 (HSP27/HSPB1) protects against cadmium-induced oxidative stress and toxicity in human cervical cancer cells; Springer; Cell Stress & Chaperones; 22; 3; 5-2017; 357-369
dc.identifier.issn
1355-8145
dc.identifier.uri
http://hdl.handle.net/11336/49788
dc.description.abstract
Cadmium (Cd) is a carcinogen with several welldescribed toxicological effects in humans, but its molecular mechanisms are still not fully understood. Overexpression of heat shock protein 27 (HSP27/HSPB1)—a multifunctional protein chaperone—has been shown to protect cells from oxidative damage and apoptosis triggered by Cd exposure. The aims of this work were to investigate the potential use of extracellular recombinant HSP27 to prevent/counteract Cdinduced cellular toxicity and to evaluate if peroxynitrite was involved in the development of Cd-induced toxicity. Here, we report that the harmful effects of Cd correlatedwith changes in oxidative stress markers: upregulation of reactive oxygen species, reduction in nitric oxide (NO) bioavailability, increment in lipid peroxidation, peroxynitrite (PN), and protein nitration; intracellular HSP27 was reduced. Treatments with Cd (100 μM) for 24 h or with the peroxynitrite donor, SIN-1, decreased HSP27 levels (~50%), suggesting that PN formation is responsible for the reduction of HSP27. Pre-treatments of the cells either with Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) (a pharmacological inhibitor of NO synthase) or with recombinant HSP27 (rHSP27) attenuated the disruption of the cellular metabolism induced by Cd, increasing in a 55 and 52%, respectively, the cell viability measured by CCK-8. Cd induced necrotic cell death pathways, although apoptosis was also activated; pre-treatment with LNAME or rHSP27 mitigated cell death. Our findings show for the first time a direct relationship between Cd-induced toxicity and PN production and a role for rHSP27 as a potential therapeutic agent that may counteract Cd toxicity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cytoprotection
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Heat Shock Protein 27 (Hsp27)
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Oxidative Stress
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Signaling Cadmiumtoxicity
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Toxicología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Recombinant heat shock protein 27 (HSP27/HSPB1) protects against cadmium-induced oxidative stress and toxicity in human cervical cancer cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-18T19:10:13Z
dc.identifier.eissn
1466-1268
dc.journal.volume
22
dc.journal.number
3
dc.journal.pagination
357-369
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Alvarez Olmedo, Daiana Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. University of Calgary; Canadá
dc.description.fil
Fil: Biaggio, Veronica Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
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Fil: Koumbadinga, Geremy A.. University of Calgary; Canadá
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Fil: Gomez, Nidia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
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Fil: Shi, Chunhua. University of Calgary; Canadá
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Fil: Ciocca, Daniel Ramon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Batulan, Zarah. University of Calgary; Canadá
dc.description.fil
Fil: Fanelli, Mariel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: O´Brien, Edward R.. University of Calgary; Canadá
dc.journal.title
Cell Stress & Chaperones
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s12192-017-0768-y
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s12192-017-0768-y
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