Artículo
TfVPS32 Regulates Cell Division in the Parasite Tritrichomonas foetus
Iriarte, Lucrecia Soledad
; Midlej, Victor; Frontera, Lorena Soledad
; Moros Duarte, Daniel Eduardo; Barbeito, Claudio Gustavo
; de Souza, Wanderley; Benchimol, Marlene; de Miguel, Natalia
; Cóceres, Verónica Mabel
Fecha de publicación:
01/2018
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Journal of Eukaryotic Microbiology
ISSN:
1066-5234
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The flagellated protist Tritrichomonas foetus is a parasite that causes bovine trichomonosis, a major sexually transmitted disease in cattle. Cell division has been described as a key player in controlling cell survival in other cells, including parasites but there is no information on the regulation of this process in T. foetus. The regulation of cytokinetic abscission, the final stage of cell division, is mediated by members of the ESCRT (endosomal sorting complex required for transport) machinery. VPS32 is a subunit within the ESCRTIII complex and here, we report that TfVPS32 is localized on cytoplasmic vesicles and a redistribution of the protein to the midbody is observed during the cellular division. In concordance with its localization, deletion of TfVPS32 C-terminal alpha helices (α5 helix and/or α4-5 helix) leads to abnormal T. foetus growth, an increase in the percentage of multinucleated parasites and cell cycle arrest at G2/M phase. Together, these results indicate a role of this protein in controlling normal cell division.
Palabras clave:
Cytokinesis
,
Escrtiii Complex
,
Vps32 Protein
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(INIMEC - CONICET)
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Citación
Iriarte, Lucrecia Soledad; Midlej, Victor; Frontera, Lorena Soledad; Moros Duarte, Daniel Eduardo; Barbeito, Claudio Gustavo; et al.; TfVPS32 Regulates Cell Division in the Parasite Tritrichomonas foetus; Wiley Blackwell Publishing, Inc; Journal of Eukaryotic Microbiology; 65; 1; 1-2018; 28-37
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