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Artículo

Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta

Diessler, Mónica Elizabeth; Ventureira, Martín RicardoIcon ; Hernández, RocíoIcon ; Sobarzo, Cristian Marcelo; Casas, L.; Barbeito, Claudio GustavoIcon ; Cebral, ElisaIcon
Fecha de publicación: 02/2017
Editorial: Wiley Blackwell Publishing, Inc
Revista: Reproduction in Domestic Animals
ISSN: 0936-6768
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

The zonary and endotheliochorial dog placenta is the most invasive placenta of carnivores. The importance of matrix metalloproteinases (MMP) in placenta invasiveness has been determined in several mammals including species with haemochorial, epitheliochorial and endotheliochorial placentation. Regarding the latter, the expression of MMP enzymes has been studied in the cat and the mature canine placenta. The aim of this study was to analyse the expression and activity of MMP-2 and MMP-9 in the early dog placenta. Placentae from 18 to 30 days of pregnancy were collected from four bitches. Two placentae from each bitch were analysed. Placental tissue from one uterine horn was fixed in formaldehyde for immunohistochemistry, while marginal haematoma, labyrinth, non-implantative and implantative endometrium from the contralateral horn were immediately frozen in dry ice for the analysis of MMP expression (Western blot [WB]) and activity (zymography). MMP-2 and MMP-9 were evidenced in the labyrinth, maternal glands and marginal haematoma; this finding was directly correlated with levels of MMP expression by WB, and with the activity of MMP-2, mainly in the haematoma (the area of major remodelling of tissues). Thus, although MMP-9 is well expressed in the early canine placenta, it is not active. Given the important role of MMPs for invasiveness, maternal–foetal angiogenesis and the establishment of a correct foetal nutrition, the results are consistent with the findings in other species in which the MMP-2 activation precedes the MMP-9 one in early placentation.
Palabras clave: Placenta , Mmp2 , Mmp9 , Canine
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/49671
DOI: https://dx.doi.org/10.1111/rda.12791
URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.12791
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Diessler, Mónica Elizabeth; Ventureira, Martín Ricardo; Hernández, Rocío; Sobarzo, Cristian Marcelo; Casas, L.; et al.; Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 52; 1; 2-2017; 35-43
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