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dc.contributor.author
Fernandez Gianotti, Tomas
dc.contributor.author
Burgueño, Adriana Laura
dc.contributor.author
Gonzales Mansilla, Noelia Luz
dc.contributor.author
Pirola, Carlos Jose
dc.contributor.author
Sookoian, Silvia Cristina
dc.date.available
2015-05-19T13:24:58Z
dc.date.issued
2013-09-30
dc.identifier.citation
Fernandez Gianotti, Tomas; Burgueño, Adriana Laura; Gonzales Mansilla, Noelia Luz; Pirola, Carlos Jose; Sookoian, Silvia Cristina; Fatty Liver Is Associated with Transcriptional Downregulation of Stearoyl-CoA Desaturase and Impaired Protein Dimerization; Public Library Science; Plos One; 8; 9; 30-9-2013; 1-11;
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/495
dc.description.abstract
AIMS AND METHODS: We evaluated the modulation of liver stearoyl-CoA desaturase-1 (Scd1) by dietary factors and insulin resistance (IR) in two experimental models of high-fat diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD). The first model included Sprague Dawley (SD) rats that developed NAFLD without IR, and the second one included a rat model of genetic IR and cardiovascular disease, the spontaneously hypertensive rats (SHR) and its normotensive, insulin-sensitive control Wistar-Kyoto (WKY). The adult rats were given standard chow diet (CD) or HFD for 10 weeks. In all the animals, we explored the hepatic Scd1 transcriptional activity and protein levels. RESULTS: HFD-fed rats of both strains developed severe NAFLD. Liver abundance of Scd1 mRNA was significantly decreased in HFD-fed rats regardless of the strain; SD-CD: 235±195 vs. SD-HFD 4.5±2.9, p<0.0004, and SHR-CD: 75.6±10.8 vs. SHR-HFD: 4.48±17.4, and WKY-CD: 168.7±17.4 vs. WKY-HFD: 12.9±17.4, p<0.000001 (mean±SE, ANCOVA adjusted by HOMA). Analysis of liver Scd1 protein expression showed a particular pattern in the HFD groups, characterized by the presence of high levels of a monomeric protein band (32.2-36.6 Kda, p<0.003) and decreased levels of a dimeric protein band (61.9-66.1 Kda, p<0.02) regardless of the rat strain. Pharmacologic intervention with the peroxisome proliferator-activated receptor α agonist clofibrate reverted the liver phenotype and significantly modified the hepatic Scd1 transcriptional activity and protein expression. CONCLUSION: Diet-induced fatty liver is associated with the downregulation of hepatic Scd1 transcript and de-dimerization of the protein, and these changes were not much affected by the status of peripheral IR.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Fatty-Liver
dc.subject.classification
Ciencias Médicas y de la Salud
dc.subject.classification
Ciencias de la Salud
dc.subject.classification
Otras Ciencias de la Salud
dc.title
Fatty Liver Is Associated with Transcriptional Downregulation of Stearoyl-CoA Desaturase and Impaired Protein Dimerization
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
8
dc.journal.number
9
dc.journal.pagination
1-11
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Fernandez Gianotti T.
dc.description.fil
Fil: Burgueño A.
dc.description.fil
Fil: Gonzales Mansilla Nl.
dc.description.fil
Fil: Pirola Cj.
dc.description.fil
Fil: Sookoian S.
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0076912


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