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dc.contributor.author Fernandez Gianotti, Tomas
dc.contributor.author Burgueño, Adriana Laura
dc.contributor.author Gonzales Mansilla, Noelia Luz
dc.contributor.author Pirola, Carlos Jose
dc.contributor.author Sookoian, Silvia Cristina
dc.date.available 2015-05-19T13:24:58Z
dc.date.issued 2013-09-30
dc.identifier.citation Fernandez Gianotti, Tomas; Burgueño, Adriana Laura; Gonzales Mansilla, Noelia Luz; Pirola, Carlos Jose; Sookoian, Silvia Cristina; Fatty Liver Is Associated with Transcriptional Downregulation of Stearoyl-CoA Desaturase and Impaired Protein Dimerization; Public Library Science; Plos One; 8; 9; 30-9-2013; 1-11;
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/11336/495
dc.description.abstract AIMS AND METHODS: We evaluated the modulation of liver stearoyl-CoA desaturase-1 (Scd1) by dietary factors and insulin resistance (IR) in two experimental models of high-fat diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD). The first model included Sprague Dawley (SD) rats that developed NAFLD without IR, and the second one included a rat model of genetic IR and cardiovascular disease, the spontaneously hypertensive rats (SHR) and its normotensive, insulin-sensitive control Wistar-Kyoto (WKY). The adult rats were given standard chow diet (CD) or HFD for 10 weeks. In all the animals, we explored the hepatic Scd1 transcriptional activity and protein levels. RESULTS: HFD-fed rats of both strains developed severe NAFLD. Liver abundance of Scd1 mRNA was significantly decreased in HFD-fed rats regardless of the strain; SD-CD: 235±195 vs. SD-HFD 4.5±2.9, p<0.0004, and SHR-CD: 75.6±10.8 vs. SHR-HFD: 4.48±17.4, and WKY-CD: 168.7±17.4 vs. WKY-HFD: 12.9±17.4, p<0.000001 (mean±SE, ANCOVA adjusted by HOMA). Analysis of liver Scd1 protein expression showed a particular pattern in the HFD groups, characterized by the presence of high levels of a monomeric protein band (32.2-36.6 Kda, p<0.003) and decreased levels of a dimeric protein band (61.9-66.1 Kda, p<0.02) regardless of the rat strain. Pharmacologic intervention with the peroxisome proliferator-activated receptor α agonist clofibrate reverted the liver phenotype and significantly modified the hepatic Scd1 transcriptional activity and protein expression. CONCLUSION: Diet-induced fatty liver is associated with the downregulation of hepatic Scd1 transcript and de-dimerization of the protein, and these changes were not much affected by the status of peripheral IR.
dc.format application/pdf
dc.language.iso eng
dc.publisher Public Library Science
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject FATTY-LIVER
dc.subject.classification Ciencias Médicas y de la Salud
dc.subject.classification Ciencias de la Salud
dc.subject.classification Otras Ciencias de la Salud
dc.title Fatty Liver Is Associated with Transcriptional Downregulation of Stearoyl-CoA Desaturase and Impaired Protein Dimerization
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.type info:eu-repo/semantics/article
dc.date.updated 2016-03-30 10:35:44.97925-03
dc.journal.volume 8
dc.journal.number 9
dc.journal.pagination 1-11
dc.journal.pais Estados Unidos
dc.journal.ciudad San Francisco
dc.description.fil Fil: Fernandez Gianotti T.
dc.description.fil Fil: Burgueño A.
dc.description.fil Fil: Gonzales Mansilla Nl.
dc.description.fil Fil: Pirola Cj.
dc.description.fil Fil: Sookoian S.
dc.journal.title Plos One
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0076912


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)