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dc.contributor.author
Dickinson, George D.
dc.contributor.author
Ellefsen, Kyle L.
dc.contributor.author
Ponce Dawson, Silvina Martha
dc.contributor.author
Pearson, John E.
dc.contributor.author
Parker, Ian
dc.date.available
2018-06-21T14:20:30Z
dc.date.issued
2016-11
dc.identifier.citation
Dickinson, George D.; Ellefsen, Kyle L.; Ponce Dawson, Silvina Martha; Pearson, John E.; Parker, Ian; Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action; American Association for the Advancement of Science; Science Signaling; 9; 453; 11-2016; 10801-10810
dc.identifier.issn
1937-9145
dc.identifier.uri
http://hdl.handle.net/11336/49523
dc.description.abstract
The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca2+-liberating secondmessenger inositol trisphosphate (IP3) diffuses with a coefficient (∼280 mm2 s-1) similar to that in water, corresponding to a range of action of ∼25 mm. Consequently, IP3 is generally considered a "global" cellular messenger. We reexamined this issue by measuring local IP3-evoked Ca2+ puffs to monitor IP3 diffusing from spot photorelease in neuroblastoma cells. Fitting these data by numerical simulations yielded a diffusion coefficient (≤10 mm2 s-1) about 30-fold slower than that previously reported. We propose that diffusion of IP3 in mammalian cells is hindered by binding to immobile, functionally inactive receptors that were diluted in oocyte extracts. The predicted range of action of IP3 (<5 mm) is thus smaller than the size of typical mammalian cells, indicating that IP3 should better be considered as a local rather than a global cellular messenger. 2016
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Association for the Advancement of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ip3
dc.subject
Calcium
dc.subject
Diffusion
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-12T16:06:36Z
dc.journal.volume
9
dc.journal.number
453
dc.journal.pagination
10801-10810
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Dickinson, George D.. University of California at Irvine; Estados Unidos
dc.description.fil
Fil: Ellefsen, Kyle L.. University of California at Irvine; Estados Unidos
dc.description.fil
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
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Fil: Pearson, John E.. Los Alamos National Laboratory; Estados Unidos
dc.description.fil
Fil: Parker, Ian. University of California at Irvine; Estados Unidos
dc.journal.title
Science Signaling
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://stke.sciencemag.org/content/9/453/ra108
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1126/scisignal.aag1625
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6876694/
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