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dc.contributor.author Surtayeva, Sofya
dc.contributor.author Velle, Katrina B.
dc.contributor.author Campellone, Kenneth G.
dc.contributor.author Talman, Arthur
dc.contributor.author Alvarez, Diego Ezequiel
dc.contributor.author Agaisse, Hervé
dc.contributor.author Wu, Yi I.
dc.contributor.author Loew, Leslie M.
dc.contributor.author Mayer, Bruce J.
dc.date.available 2018-06-21T12:48:28Z
dc.date.issued 2016-01
dc.identifier.citation Surtayeva, Sofya; Velle, Katrina B.; Campellone, Kenneth G.; Talman, Arthur; Alvarez, Diego Ezequiel; et al.; Integration of linear and dendritic actin nucleation in Nck-induced actin comets; American Society for Cell Biology; Molecular Biology Of The Cell; 27; 2; 1-2016; 247-259
dc.identifier.issn 1059-1524
dc.identifier.uri http://hdl.handle.net/11336/49516
dc.description.abstract The Nck adaptor protein recruits cytosolic effectors such as N-WASP that induce localized actin polymerization. Experimental aggregation of Nck SH3 domains at the membrane induces actin comet tails-dynamic, elongated filamentous actin structures similar to those that drive the movement of microbial pathogens such as vaccinia virus. Here we show that experimental manipulation of the balance between unbranched/branched nucleation altered the morphology and dynamics of Nck-induced actin comets. Inhibition of linear, form-in-based nucleation with the small-molecule inhibitor SMIFH2 or overexpression of the formin FH1 domain resulted in formation of predominantly circular-shaped actin structures with low mobility (actin blobs). These results indicate that formin-based linear actin polymerization is critical for the formation and maintenance of Nck-dependent actin comet tails. Consistent with this, aggregation of an exclusively branched nucleation-promoting factor (the VCA domain of N-WASP), with density and turnover similar to those of N-WASP in Nck comets, did not reconstitute dynamic, elongated actin comets. Furthermore, enhancement of branched Arp2/3-mediated nucleation by N-WASP overexpression caused loss of the typical actin comet tail shape induced by Nck aggregation. Thus the ratio of linear to dendritic nucleation activity may serve to distinguish the properties of actin structures induced by various viral and bacterial pathogens.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Society for Cell Biology
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject NUCLEATION PROMOTING FACTOR
dc.subject SRC HOMOLOGY 3
dc.subject VACCINIA VIRUS
dc.subject FORMIN
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Integration of linear and dendritic actin nucleation in Nck-induced actin comets
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-06-19T16:51:25Z
dc.journal.volume 27
dc.journal.number 2
dc.journal.pagination 247-259
dc.journal.pais Estados Unidos
dc.journal.ciudad Bethesda
dc.description.fil Fil: Surtayeva, Sofya. University of Connecticut School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Velle, Katrina B.. University of Connecticut; Estados Unidos
dc.description.fil Fil: Campellone, Kenneth G.. University of Connecticut; Estados Unidos
dc.description.fil Fil: Talman, Arthur. Yale School of Medicine; Estados Unidos
dc.description.fil Fil: Alvarez, Diego Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil Fil: Agaisse, Hervé. Yale School of Medicine; Estados Unidos
dc.description.fil Fil: Wu, Yi I.. University of Connecticut School of Medicine; Estados Unidos
dc.description.fil Fil: Loew, Leslie M.. University of Connecticut School of Medicine; Estados Unidos
dc.description.fil Fil: Mayer, Bruce J.. University of Connecticut School of Medicine; Estados Unidos
dc.journal.title Molecular Biology Of The Cell
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1091/mbc.E14-11-1555
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.molbiolcell.org/doi/10.1091/mbc.e14-11-1555
dc.conicet.fuente individual


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    Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS

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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)