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dc.contributor.author
Surtayeva, Sofya
dc.contributor.author
Velle, Katrina B.
dc.contributor.author
Campellone, Kenneth G.
dc.contributor.author
Talman, Arthur
dc.contributor.author
Alvarez, Diego Ezequiel
dc.contributor.author
Agaisse, Hervé
dc.contributor.author
Wu, Yi I.
dc.contributor.author
Loew, Leslie M.
dc.contributor.author
Mayer, Bruce J.
dc.date.available
2018-06-21T12:48:28Z
dc.date.issued
2016-01
dc.identifier.citation
Surtayeva, Sofya; Velle, Katrina B.; Campellone, Kenneth G.; Talman, Arthur; Alvarez, Diego Ezequiel; et al.; Integration of linear and dendritic actin nucleation in Nck-induced actin comets; American Society for Cell Biology; Molecular Biology Of The Cell; 27; 2; 1-2016; 247-259
dc.identifier.issn
1059-1524
dc.identifier.uri
http://hdl.handle.net/11336/49516
dc.description.abstract
The Nck adaptor protein recruits cytosolic effectors such as N-WASP that induce localized actin polymerization. Experimental aggregation of Nck SH3 domains at the membrane induces actin comet tails-dynamic, elongated filamentous actin structures similar to those that drive the movement of microbial pathogens such as vaccinia virus. Here we show that experimental manipulation of the balance between unbranched/branched nucleation altered the morphology and dynamics of Nck-induced actin comets. Inhibition of linear, form-in-based nucleation with the small-molecule inhibitor SMIFH2 or overexpression of the formin FH1 domain resulted in formation of predominantly circular-shaped actin structures with low mobility (actin blobs). These results indicate that formin-based linear actin polymerization is critical for the formation and maintenance of Nck-dependent actin comet tails. Consistent with this, aggregation of an exclusively branched nucleation-promoting factor (the VCA domain of N-WASP), with density and turnover similar to those of N-WASP in Nck comets, did not reconstitute dynamic, elongated actin comets. Furthermore, enhancement of branched Arp2/3-mediated nucleation by N-WASP overexpression caused loss of the typical actin comet tail shape induced by Nck aggregation. Thus the ratio of linear to dendritic nucleation activity may serve to distinguish the properties of actin structures induced by various viral and bacterial pathogens.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Cell Biology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Nucleation Promoting Factor
dc.subject
Src Homology 3
dc.subject
Vaccinia Virus
dc.subject
Formin
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Integration of linear and dendritic actin nucleation in Nck-induced actin comets
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-19T16:51:25Z
dc.journal.volume
27
dc.journal.number
2
dc.journal.pagination
247-259
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Bethesda
dc.description.fil
Fil: Surtayeva, Sofya. University of Connecticut School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Velle, Katrina B.. University of Connecticut; Estados Unidos
dc.description.fil
Fil: Campellone, Kenneth G.. University of Connecticut; Estados Unidos
dc.description.fil
Fil: Talman, Arthur. Yale School of Medicine; Estados Unidos
dc.description.fil
Fil: Alvarez, Diego Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil
Fil: Agaisse, Hervé. Yale School of Medicine; Estados Unidos
dc.description.fil
Fil: Wu, Yi I.. University of Connecticut School of Medicine; Estados Unidos
dc.description.fil
Fil: Loew, Leslie M.. University of Connecticut School of Medicine; Estados Unidos
dc.description.fil
Fil: Mayer, Bruce J.. University of Connecticut School of Medicine; Estados Unidos
dc.journal.title
Molecular Biology Of The Cell
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1091/mbc.E14-11-1555
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.molbiolcell.org/doi/10.1091/mbc.e14-11-1555


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